Tamay DL, Ibarra C, Velasquillo C

Language: Spanish
References: 13
Page: 70-78
PDF size: 329.72 Kb.

ABSTRACT

After three decades of discovering utility of polymerase chain reaction, its methodology has become one of the most employed tools for the study of nucleic acids. This is a top sensitive, highly reproductible, very efficient and short time consuming technology that allows to obtain reliable and easy to evaluate results. Objectives of this paper deal with fundamentals of both techniques standard polymerase chain reaction as well as the polymerase chain reaction on real time. Results produced by standard polymerase chain reaction are qualitative, while those produced by polymerase chain reaction on real time are quantitative.

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