González-Quevedo MA, Fernández CR, León OMM, González GS, VicenteVI

Language: Spanish
References: 27
Page: 242-247
PDF size: 154.94 Kb.

ABSTRACT

Introduction and objective: Cerebrospinal (CSF) protein fractioning is a basic tool for the diagnosis and follow-up of some neurological diseases, thus information on the normal electrophoretic patterns is essential. The objective of this work is to present the results obtained in our laboratory for normal CSF from children and adults. Methods: 7.5% polycrylamide gel electrophoresis was employed for serum and CSF protein fractioning in 109 children and 55 adults of both genders. Gels were stained with Coomassie Brilliant Blue to obtain the general protein pattern and with NADI reagent to reveal serum haptoglobin bands. Results and conclusions: The CSF protein concentration and the gamma fraction were lower, and the prealbumin-1 fraction was higher in children than in adults. The presence of haptoglobin bands in the CSF was not indicative of increased permeability of the blood-CSF barrier, as they were encountered in 65.7% of children and in 79.6% of adults. The technique employed allowed the acquirement of rapid and precise information, which is very useful for the diagnostic support and followup of some diseases of the nervous system and has been the basis for the interpretation of pathological electrophoretic patterns in our laboratory.

REFERENCES

1. Reiber H, Peter JB. Cerebrospinal fluid analysis: disease-related data patterns and evaluation programs. Journal of the Neurological Sciences 2001; 184: 101-22.

2. Fishman RA (ed.). Cerebrospinal fluid in diseases of the nervous system. 2nd. Ed. Philadelphia, Pennsylvania, W.B. Saunders Company; 1992.

3. Siegel GF, Agranoff BW, Albers RW, Fisher SK, Uhler MD (eds.). Blood-Brain-Cerebrospinal Fluid Barriers. In: Basic Neurochemistry. Lippincot-Raven Publishers; 1999.

4. Thompson EJ (ed.). The CSF proteins: a biochemical approach, Elsevier; 1988.

5. Saunders NR, Habgood MD, Dziegielewska KM. Barrier mechanisms in the brain. I. Adult brain. Clin Exp Pharmacol Physiol 1999; 26: 11-19.

6. Massaro AR, Scivoletto G, Tonali P. Cerebrospinal fluid markers in neurological diseases. Ital J Neurol Sci 1990; 11(6): 537-47.

7. Takeoka T, Shinohara Y, Furumi K, Mori K. Characteristic protein fractions of cerebrospinal fluid disc electrophoresis analysis. Brain Res 1980; 198: 147-56.

8. Alekseeva LA, Sorokina MN. Diagnostic significance of the cerebrospinal fluid protein spectrum in bacterial and viral meningitis in children. Klin Lab Diagn 2001; 7: 15-19.

9. Burcet J, Zabay JM, Usón M, Mulet J, Figuerola A, Viander C. Síntesis Intratecal de IgG valorada mediante diferentes fórmula: Interés del análisis multivariante para el diagnóstico de esclerosis múltiple. Rev Neurol 2000; 31(9): 812-16.

10. Masjuan J, Alvarez-Cermeño JC, García-Barragán N, Díaz-Sánchez M, Espiño M, Sádaba MC, et al. Clinically isolated syndromes. A new oligoclonal band test accurately predicts conversion to MS. Neurology 2006; 66: 576-8.

11. Evans JH, Quick DT. Polyacrylamide gel electrophoresis of spinal fluid proteins. Arch Neurol 1966; 14: 64-71.

12. Cerda M, Vielma J, Basauri L. Electroforesis en gel de poliacrilamida de proteínas del líquido cefalorraquídeo. Neurocirugía 1978; 36(4): 330-3.

13. Fernández CR, González-Quevedo A, Lara RR, León OMM, González GS, Valdés IV. Electroforesis de proteínas del líquido cefalorraquídeo en pacientes con enfermedad cerebrovascular isquémica. Rev Neurol 2002; 35(10): 908-12.

14. Lowry OH, Rosebrugh RJ, Farr Al, Randal RJ. Protein measurement with Folin phenol reagent. J Biol Chem 1951; 193: 265-75.

15. Smith I (ed.). Chromatographic and electrophoretic techniques. Vol. II. Zone electrophoresis. Great Britain: Pitman Press; 1968.

16. Grabar P, Bartin P (eds.). Inmunoelectroforesis. Barcelona: Ed. Toray- Masson; 1968.

17. Seyfert S, Kunzmann V, Schwertfeger N, Koch HC, Faulstich A. Determinants of lumbar CSF protein concentration. J Neurol 2002; 249: 1021-6.

18. Freedman MS, Thompson EJ, Deisenhammer F, Giovannoni G, Grimsley G, Keir G, et al. Recommended Standard of Cerebrospinal Fluid Analysis in the Diagnosis of Multiple Sclerosis. Arch Neurol 2005; 62: 865-70.

19. Biou D, Benoist JF, Nguyen-Thi C, Huong X, Morel P, Marchand M. Cerebrospinal fluid protein concentrations in children: Age-related values in paients without disorders of the nervous system. Clin Chem 2000; 46(3): 399-403.

20. Saunders NR, Habgood MD, Dziegielewska KM. Barrier mechanisms in the brain, II. Immature brain. Clin Exp Pharmacol Physiol 1999; 26: 85-91.

21. Johansson PA, Dziegielewska KM, Ek CJ, Habgood MD, Liddelow SA, Potter AM, Stolp HB Saunders NR. Blood-CSF barrier function in the rat embryo. J Neuroscience 2006; 24: 65-76.

22. Tibbling G, Link H, Ohman S. Principles of albumin and IgG analysis in neurological disorders. I. Establishment of reference values. Scand J Clin Lab Invest 1977; 37: 385-90.

23. Gerbaut L, Ponsot G (eds.). Interpretation of the protein profile of cerebrospinal fluid in children. In: Marker proteins in Inflammation. 1984; 2: 369-73.

24. Cerda M, Vielma J, Martinez C, Basauri L. Polyacrylamide gel electrophoresis of cerebrospinal fluid proteins in children with nontumoral hydrocephalus. Child’s Brain 1980; 6: 140-9.

25. Weisner B, Roethig HJ. The concentration of prealbumin in the cerebrospinal fluid (CSF), indicator of CSF circulation disorders. Eur Neurol 1983; 22: 96-105.

26. Strand T, Alling C, Karlsson B, Karlsson I, Winblad B. Brain and plasma proteins in spinal fluid as markers for brain damage and severity of stroke. Stroke 1984; 15(1): 138-44.

27. Chamoun V, Zeman A, Blennow K, Fredman P, Wallin A, Keir G, et al. Haptoglobins as markers of blood-CSF barrier dysfunction: the findings in the normal CSF. J Neurol Sci 2001; 182(2): 117-21.