Escobar HED, de Armas RY, Cantelar FN, Capó PV, Cathcart RF, Jiménez LMA

Language: Spanish
References: 12
Page: 294-301
PDF size: 97.53 Kb.

ABSTRACT

Introduction: Helicobacter pylori is considered one of the main causal agents of chronic gastritis, peptic ulcer and gastric malignant neoplasms in humans.
Objective: to evaluate polymerase chain reaction for identification of Helicobacter pylori and its genotypes in paraffin embedded gastric tissues with malignant neoplasms.
Material and Methods: sections of five paraffin blocks from five patients with gastric malignant neoplasms were studied. They were analyzed through routine and special stains of pathological anatomy, as well as the polymerase chain reaction technic for microorganism and genotypes detection.
Results: the infectious agent was identified in all of the analyzed blocks through the polymerase chain reaction technic in correspondence with its detection through histologic techniques. This methodology showed a genetic variability of the pathogen in the analyzed samples in respect to vacA and cagA genotypes.
Conclusions: the polymerase chain reaction could be an efficacious method for the identification of H. pylori in paraffin embedded gastric tissues with malignant neoplasms. It is projected as an attractive strategy for performing studies of molecular epidemiology and the establishment of possible associations between genotypes/subtypes of the microorganism and clinic or epidemiologic variables, and patient handling.

REFERENCES

1. Ferreira RM, Machado JC, Letley D, Atherton JC, Pardo ML, Gonzalez CA, et al. A novel method for genotyping the Helicobacter pylori vacA intermediate region directly in gastric biopsy specimens. J Clin Microbiol.2012;50(12):3983-9.

2. Ciesielska U, Dziegiel P, Jagoda E, Podhorska-Oko³ów M, Zabel M. The detection of Helicobacter pylori in paraffin sections using the PCR technique and various primers as compared to histological techniques. Folia Morphol (Warsz). 2004;63(2):229-31.

3. Vela-Velázquez CT. Comparación entre las biopsias gástricas sin fijar 24 horas frente a la biopsia convencional para el diagnóstico de Helicobacter pylori en un hospital de referencia de Perú. Rev Peru Med Exp Salud Pública. 2011;28(1): 42-6.

4. Sicinschi LA, Correa P, Peek Jr RM, Constanza Camargo M, Delgado A, Piazuelo MB, et al. Helicobacter pylori genotyping and sequencing using paraffin-embedded biopsies from residents of colombian areas with contrasting gastric cancer risks. Helicobacter. 2008;13(2):13545.

5. Farivar TN, Pahlevan A, Johari P, Safdarian F, Mehr MA, Najafipour R, et al. Assessment of Helicobacter pylori prevalence by scorpion real-time PCR in chronic tonsillitis patients. J Glob Infect Dis. 2012; 4(1):38-42.

6. Han HS, Lee KY, Lim SD, Kim WS, Hwang TS. Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis. Oncol Lett. 2010;1(3):555-8.

7. Koehler CI, Mues MB, Dienes HP, Kriegsmann J, Schirmacher P, Odenthal M. Helicobacter pylori genotyping in gastric adenocarcinoma and MALT lymphoma by multiplex PCR analyses of paraffin wax embedded tissues. Mol Pathol. 2003;56(1):36-42.

8. López-Vidal Y, Ponce-de-León S, Castillo-Rojas G, Barreto-Zúñiga R, Torre- Delgadillo A. High diversity of vacA and cagA Helicobacter pylori genotypes in patients with and without gastric cancer. PLoS ONE. 2008; 3(12):e3849.

9. González-Vázquez R, Herrera-González S, Córdova-Espinoza MG, Zúñiga G, Giono-Cerezo S, Hernández-Hernández JM, et al. Helicobacter pylori: detection of iceA1 and iceA2 genes in the same strain in Mexican isolates. Arch Med Res. 2012;43(5): 339-46.

10. Miciuleviciene J, Calkauskas H, Jonaitis L, Kiudelis G, Tamosiûnas V, Praskevicius A, et al. Helicobacter pylori genotypes in Lithuanian patients with chronic gastritis and duodenal ulcer. Medicina (Kaunas). 2008;44(6):449-54.

11. Paniagua GL, Monroy E, Rodríguez R, Arroniz S, Rodríguez C, Cortés JL, et al. Frequency of vacA, cagA and babA2 virulence markers in Helicobacter pylori strains isolated from Mexican patients with chronic gastritis. Ann Clin Microbiol Antimicrob. 2009; 8:14. doi: 10.1186/1476-0711-8-14.

12. Armas Y, Capó V, López L, Mederos L, Díaz R. Comparación de tres métodos de extracción de tejidos embebidos en parafina. Biotecn Apl. 2011; 28(1):40-3.