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2013, Number 2

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Revista Habanera de Ciencias Médicas 2013; 12 (2)

Diabetic embryopathy in rats and effect of a nutritional supplement of vitamin e during gestation

Fernández RT, Clapés HS, Suárez RG, Perera CA, Rodríguez SVM, Purón GCA, Herrera ÁM, Antiguas PÁ, Castro MT

Language: Spanish
References: 31
Page: 176-186
PDF size: 128.43 Kb.


ABSTRACT

Introduction: experimental models have obtained good results with the supplementation of antioxidants in the prevention of the diabetic embryopathy. However, not always the effect is beneficial and the mechanism of these results is unclear. T
Objective: was to evaluate the effect of nutritional supplementation with vitamin E during gestation on offspring of diabetic rats.
Materials and Methods: we used wistar rats with diabetes induced by streptozotocin and health rats like controls. During gestation, one group of diabetic rats and one group of controls received vitamin E 150mg/kg/day, and the others group the vehicle. Euthanasia was practiced at 11,5 day of pregnancy and we study the morphology of the products, DNA, proteins and oxidative stress markers in embryos. The statistic analyze was performed with non parametric tests and the significant differences were considered with p ‹ 0, 05.
Results: in diabetic rats with vitamin E the embryos were higher and decreased the number of resorptions, the developmental impairment and the content of oxidative damage markers to lipids and proteins in the embryos.
Conclusions: nutritional supplementation with vitamin E during gestation in diabetic rats decreased the loss of the conception products, increased the growth and development of the embryos with decreased oxidative damage to biomolecules that will be suggest a beneficial antioxidant effect of the vitamin.


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