medigraphic.com
Órgano Oficial de la Asociación Mexicana de Infectología Pediátrica y la SLIPE.

2011, Number 96

<< Back Next >>

Rev Enfer Infec Pediatr 2011; 24.25 (96)

Genetic identification of strains of Aeromonas spp isolated at the National Institute of Pediatrics

Hernández-Cortez C, Aguilera-Arreola MG, Chávez- Méndez Y, Arzate-Barbosa P, Lara-Hernández A, Castro- Escarpulli G

Language: Spanish
References: 37
Page: 162-171
PDF size: 288.57 Kb.


ABSTRACT

Background: Aeromonas species cause intestinal and extraintestinal infections in humans. Phenotypic methods do not always allow to identify them correctly.
Objective: To identify a group of genetically engineered strains of Aeromonas spp. isolated mainly from children with intestinal and extraintestinal infections.
Material and methods: The 69 strains of this study were previously isolated and identified in the National Institute of Pediatrics for the MicroScan Walk Away system 96 (W/A 96). The 69 strains were re-identified using the method of RFLP-PCR of 16S rDNA gen.
Results: There was found a very poor correlation percent between both methods (28.9%). Aeromonas caviae was the most isolated. The lower correlation between phenotypic and genotypic method was to identify A. caviae and Aeromonas hydrophila with the system MicroScan W/A 96.
Conclusions: The results show the need to use the genetic approach to recognize the identity of species that cause diseases in the Mexican population and thus provide clear data on the epidemiology of this genus.


REFERENCES

  1. Janda JM, Abbott SL. Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations, and unanswered questions. Clin Infect Dis 1998;27:332-344.

  2. Figueras MJ, Suarez-Franquet A, Chacón MR, Soler L, Navarro M, et al. First record of the rare species Aeromonas culicicola from a drinking water supply. Appl Environ Microbiol 2007;71:538-541.

  3. von Graevenitz A. The role of Aeromonas in diarrhea: a review. Infection 2007;35:59-64.

  4. Martin-Carnahan A, Joseph SW. Aeromonadaceae. Bergey’s Manual of Systematic Bacteriology. 2nd edition. New York: Springer, 2005; vol. 2.

  5. Janda, JM, Abbott SL. The Genus Aeromonas: Taxonomy, Pathogenicity, and Infection. Clin Microbiol Rev 2010;23:35-73.

  6. Martínez-Murcia AJ, Benlloch S, Collins MD. Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S r bosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int J Syst Bacteriol 1992;42:412-421.

  7. Dijkshoorn L, Towner KJ, Struelens M. In: New approaches for the generation and analysis of microbial typing data. Chapter 1. An introduction to the generation and analysis of microbial typing data. New York: Elsevier Science, 2001;2-25

  8. Abbot S, Seli LS, Catino M, Hartley MA, Janda M. Misidentification of unusual Aeromonas species as members of the genus Vibrio: a continuing problem. J Clin Microbiol 1998;36:1103-1104.

  9. Borrel N, Acinas S, Figueras MJ, Martínez-Murcia AJ. Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S RNA genes. J Clin Microbiol 1997;35:1671-1674.

  10. Vivas J, Saa AI, Tinajas A, Barbeyto L, Rodriguez LA. Identification of motile Aeromonas strains with the MicroScan Wa kAway system in conjunction with the combo negative type 1S panels. App Environ Microbiol 2000;66:1764-1766.

  11. Figueras MJ, Soler L, Chacón MR, Guarro J, Martínez-Murcia AJ. Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP. Int Syst Evol Microbiol 2000;50:2069-2073.

  12. Alperi A, Figueras MJ, Inza I, Martínez-Murcia AJ. Analysis 16S rRNA gene mutations in a subset of Aeromonas strains and their impact in species delineation. International Microbiology 2008;11:185- 194.

  13. National Center for Biotechnology Information. USA: http://www. ncbi.nlm.nih.gov

  14. Castro-Escarpulli G, Aguilera MG, Giono S, Hernández CH, y col. El género Aeromonas. ¿Un patógeno importante en México? Enf Infec Microb 2002;22:206-216.

  15. Guevara JM, Huamaní C, Zerpa R, Valencia E, Guevara JMM, Anaya M. Aeromonas en la diarrea aguda de niños menores de 5 años. Anales de la Facultad de Medicina 2002;63:125-129.

  16. A bert MJ, Ansaruzzaman M, Talukder KA, Chopra AK, et al. Prevalence of enterotoxin genes in Aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment. J Clin Microbiol 2000;38:3785-3790.

  17. Hänninen ML, Hirvelä-Koski V. Genetic diversity of atypical Aeromonas salmonicida studied by pulsed-field gel electrophoresis. Epidemiol Infect 1999;123:299-307.

  18. Figueras MJ, Soler L, Martinez-Murcia A, Guarro J, Aeromonas bestiarum and A. salmonicida, two closely related species of clinical significance. Sixth International Aeromonas/Plesiomonas Symposium, Chicago, USA 1999. P:17.

  19. Carnahan AM, Joseph SW. Systematic assessment of geographically and clinically diverse aeromonads. Syst Appl Microbiol 1993;16:72-84.

  20. Aguilera-Arreola MG, Hernández-Rodríguez CH, Castro-Escarpulli G. Molecular and phenotypic characterization of A. hydrophilalike HG3 strain isolated of an infant with diarrhea in Mexico. Bioquimia 2009;34: 183-189.

  21. Castro-Escarpulli G, Figueras M.J, Aguilera-Arreola MG, Soler L, Fernández-Rendón E, Aparicio GO, Guarro JM, Chacón R. Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico. Inter J Food Microbiol 2003;84:41– 49.

  22. Szczuka E, Kaznowski A. Typing of Clinical and Environmental Aeromonas sp. Strains by Random Amplified Polymorphic DNA PCR, Repetitive Extragenic Palindromic PCR, and Enterobacterial Repetitive Intergenic Consensus Sequence PCR. J Clin Microbiol 2004;42:220–228.

  23. Janda JM. Aeromonas and Plesiomonas. In: Molecular Medical Microbiology. San Diego: Academic Press, 2001;1237-1270.

  24. Cabrera L, Castro-Escarpulli G, Ramirez M, Llop A, y col. Aislamiento e identificación de especies pertenecientes a los géneros Aeromonas, Vibrio y Plesiomonas procedentes de muestras extraintestinales en Cuba. Rev Chil Infect 2007;24:204-208.

  25. Tena D, González-Praetarius A, Gimeno C, Pérez-Pomata MT, Bisquert J. Infección Extraintestinal por Aeromonas spp: revisión de 38 casos. Enferm Infecc Microbiol Clin 2007;25:235-241.

  26. Ojeda-Vargas M, González-Fernández MA, Alfonso-Rodríguez O, Monzón-Moreno C. Infecciones del tracto urinario causadas por bacterias del género Aeromonas. Enferm Infecc Microbiol Clin 2005;23:179-182.

  27. Parras F, Diaz MD, Reina J, Moreno S, Guerrero C, Bouza E. Meningitis due to Aeromonas species: Case report and review. Clin Infec Dis 1993;17:1058-1060.

  28. Camus AC, Durborow RM, Hemstreet WG, Thune RL, Hawke JP. Aeromonas Bacterial Infections-Motile Aeromonad septicemia. SRAC 1998;478:1-4.

  29. Wilcox MH, Cook AM, Eley A, Spencer RC. Aeromonas spp. as a potential cause of diarrhea in children. J Clin Pathol 1992;45:959- 963.

  30. Abbot S, Seli LS, Catino M, Hartley MA, Janda M. Misidentification of unusual Aeromonas species as members of the genus Vibrio: a continuing problem. J Clin Microbiol 1998;36:1103-1104.

  31. Janda JM, Abbot SL, Khashe S, Kellog GH, Shimada T. Further studies on biochemical characteristics and serologic properties of the genus Aeromonas. J Clin Microbiol 1996;34:1930-1933.

  32. Castro-Escarpulli G, Aguilera MG, Hernández CH, Arteaga RI, y col. La identificación genética de Aeromonas, una realidad y una necesidad para la microbiología diagnóstica. Bioquimia 2003;28:11- 18.

  33. Soler L, Marco F, Vila J, Chacon MR, et al. Evaluation of two miniaturized systems, MicroScan W/A and BBL Crystal E/NF, for identification of clinical isolates of Aeromonas spp. J Clin Microbiol 2003;41:5732-5734.

  34. Aquino-Santiago C, Lugo-de la Fuente G, Castañeda-Colín C, Guerrero MER, y col. Programa de Evaluación de la calidad entre laboratorios. XXVIII. Diez Evaluaciones de Calidad analítica en Bacteriología Médica. III. Lab Acta 2002;14:21-27.

  35. Vivas J, Saa AI, Tinajas A, Barbeyto L, Rodriguez LA. Identification of motile Aeromonas strains with the MicroScan WalkAway system in conjunction with the combo negative type 1S panels. App Environ Microbiol 2000;66:1764-1766.

  36. Castro-Escarpulli G, Aguilera-Arreola MG, Giono-Cerezo S. Capítulo XXII Género Aeromonas. En Molina J. Bacteriología y Virología Médica. 1a. ed. México: Méndez Editores, 2010; 261-274.

  37. Horneman AJ, Ali A, Abbott SL. Aeromonas. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yueken RH. Manual of Clinical Microbiology. 9th edition. Washington: American Society for Microbiology, 2007; 716-722.




2020     |     www.medigraphic.com