2012, Number 4
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Vet Mex 2012; 43 (4)
Clinical efficacy of sodium alginate meshes for the control of infectious dissemination by Escherichia coli and Staphylococcus epidermidis in cavitated wounds with primary healing
Luna del Villar VJ, Bernard BMJ, Tapia PG, Gutiérrez OL, Sumano LH
Language: English/Spanish
References: 26
Page: 285-293
PDF size: 186.08 Kb.
ABSTRACT
The potential wound healing ability of sodium alginate (SA) meshes was determined in cavitated rat wounds infected either
with
Escherichia coli or
Staphylococcus epidermidis. Wound progress was evaluated macroscopically and histopathologically
to assess dissemination of the induced infection through evaluation of granuloma formation and adjacent tissue irritation.
Five groups of 24 female Wistar rats weighing 280 ± 34 g were formed as follows: control group with SA mesh
without inoculum, control group with
Escherichia coli inoculum without SA mesh, control group with
Staphylococcus epidermidis
inoculum without mesh, group with SA mesh + Escherichia coli inoculum and group with SA mesh +
Staphylococcus
epidermidis inoculum. Abdominal flank incisions were performed under anaesthesia and groups were formed.
Both macroscopic and histopathological follow up were carried out and results indicated that the presence of SA meshes
avoids or greatly diminishes bacteria dissemination and adjacent tissue irritation. This reaction occurs even in the presence
of
Escherichia coli or
Staphylococcus epidermidis. In the control groups with SA mesh without inoculum and in the
groups with
Escherichia coli inoculum without SA mesh, and control group with
Staphylococcus epidermis inoculum without
mesh and group with SA mesh +
Escherichia coli inoculum re-epithelialization and wound healing aspect were better than
in groups inoculated with
Staphylococcus epidermidis, the general odd ratios showed that the dissemination risk was 58
times greater in treatment with SA mesh and
E. coli as infectious agent (IC 95% 6.2-210). These results indicate that SA mesh is capable of providing adequate conditions to allow granulation of cavitated wounds, without irritation to adjacent
tissues and avoiding dissemination of the infection by
E. coli and
Staphylococcus epidermidis in rats. However, presence
of granuloma can be a healing or esthetic disadvantage, as observed in control group with SA mesh without inoculum.
REFERENCES
SLATTER DH. Textbook of small animal surgery. 2nd ed. Philadelphia, USA: Saunders WB, 2003.
KAISER AB, KERNODLE DS, PARKER RA. Low- Inoculum Model of Surgical Wound Infection. J Infect Dis 1992; 166: 393-399.
ORSINI J. Wound infections and antimicrobial therapy. In: GOURLEY IM, VASSEUR PB, editors. General Small Animal Surgery. Philadelphia: JB Lippincott, 1985:121.
PEPPAS NA, SCOTT JE. Controlled release from poly (vinyl alcohol) gels prepared by freezing-thawing processes. J Control Release 1992; 18: 95-100.
KILI S, TÜMURKAAN N, NSALDI S, GÜNAY C, ÜSTEK Z, YILMAZ B. Comparison of the effects of some wound healing materials on full thickness skin wounds in rabbits. Turk J Vet Anim Sci 2002; 26: 263-272.
UEYAMA A Y, ISHIKAWA B K, MANOC T, KOYAMA C T, NAGATSUKA D H, SUZUKI B K et al. Usefulness as guided bone regeneration membrane of the alginate membrane. Biomaterials 2002; 23: 2027-2033.
COHEN SB, MEIRISCH CM, WILSON HA, DIDUCH DR. The use of absorbable co-polymer pads with alginate and cells for articular cartilage repair in rabbits. Biomaterials 2003; 24: 2653-2660.
LEOR J, TUVIA S, GUETTA V, MANCZUR F, CASTEL D, WILLENZ U et al. Intracoronary injection of in situ forming alginate hydrogel reverses left ventricular remodeling after myocardial infarction in swine. J Am Coll Cardiol 2009; 54: 1014-1023.
CHOI YS, HONG SR, LEE YM, SONG KW, PARK MH, NAM YS. Study on gelatin-containing artificial skin: I. Preparation and characteristics of novel gelatin- alginate sponge. Biomaterials 1999; 20: 409-417.
THOMAS AKG, MOORE HK. Alginates from wound dressings activate human macrophages to secrete tumour necrosis factor-α. Biomaterials 2000; 21: 1797-1802.
MURAKAMI K, AOKI H, NAKAMURA S, NAKA- MURA S-I, TAKIKAWA M, HANZAWA M et al. Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials 2010; 31: 83-90.
BALAKRISHNANA B, MOHANTYB M, UMASHAN- KARC PR, JAYAKRISHNANA A. Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin. Biomaterials 2005; 26: 6335-6342.
HONG H-J, JIN S-E, PARK J-S, AHN WS, KIM C-K. Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate poly- electrolyte complex. Biomaterials 2008; 29: 4831-4837.
GROVES AR, LAWRENCE JC. Alginate dressing as a donor site haemostat. Ann R Coll Surg Engl 1986; 68: 1-2.
KIM JO, PARK JK, KIM JH, JIN SG, YONG CS, LI DX et al. Development of polyvinyl alcohol-sodium alginate gel-matrix-based wound dressing system containing nitrofurazone. Int J Pharm 2008; 359: 79-86.
HASHIMOTO T, SUZUKI Y, TANIHARA M, KAKI- MARU Y, SUZUKI K. Development of alginate wound dressings linked with hybrid peptides derived from laminin and elastin. Biomaterials 2004; 25: 1407-1414.
ALINE SA. Animales de laboratorio y la Norma Oficial Mexicana (NOM-062-ZOO-1999). Gaceta Médica Mexicana 2002; 138: 295-298.
RAYMOND CR, PAUL JS, SIÂN CO. Handbook of Pharmaceutical Excipients. 5th ed. London UK: Pharmaceutical Press, 2006.
HERDT TH. Fisiología y metabolismo gastrointestinal. En: CUNNINGHAM JG, KLEIN BG, editores. Fisiología Veterinaria. 4ª ed. Barcelona, España: Elsevier España, 2009: 300-310.
CARPENTER WC. Formulario de animales exóticos. 3ª ed. México DF: Intermédica, 2006.
HEAGERTY PJ, ZEGER SL. Marginal Regression Models for clustered Ordinal Measurements. J Am Stat Assoc 1996; 91: 1024-1036.
MATTHEW IR, BROWNE RM, FRAME JW, MILLAR BM. Subperiosteal behaviour of alginate and cellulose wound dressing materials. Biomaterials 1995; 16: 265-74.
PELUSO G, PETILLO O, RANIERI M, SANTIN M, AMBROSIO L, GALABRO D et al. Chitosan-mediated stimulation of macrophage function. Biomaterials 1994; 15: 1215-20.
UBEDA C, TORMO MA, CUCARELLA C, TROTONDA P, FOSTER TJ, LASA I et al. Sip, an integrase protein with excision, circularization and integration activities, defines a new family of mobile Staphylococcus aureus pathogenicity islands. Mol Microbiol 2003; 49: 193-210.
STUART LM, DENG J, SILVER JM, TAKAHASHI K, TSENG AA, HENNESSY EJ et al. Response to Staphylococcus aureus requires CD36-mediated phagocytosis triggered by the COOH-terminal cytoplasmic domain. J Cell Biol 2005; 170: 477-485.
DORIA-SERRANO MC, RUIZ-TREVIÑO FA, RIOS- ARCIGA C, HERNÁNDEZ-ESPARZA M, SANTIAGO P. Physical characteristics of poly (vinyl alcohol) and calcium alginate hydrogels for the immobilization of activated sludge. Biomacromolecules 2001; 2: 568-574.
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