López ME, Vidal SF, Núñez FRM, Piña OV, Lugo ZG, Delgado OMD

Language: Spanish
References: 21
Page: 260-263
PDF size: 29.01 Kb.

ABSTRACT

Introduction. The rheumatoid arthritis (RA) is a disorder that affects one or more joints in a progressive and incapacitated way. The pathogenesis of the RA is related to the molecules involved in the inflammation and to the human leukocyte antigen (HLA). The latter is classified into three types: the type I is divided in HLA-A and HLA-B; the type II, HLA-DR, HLA.DP, HLA-DQ, and the type III is formed by genes that take part in both, the complement pathway (C1-C6) and the TNF. The association between the HLA and the RA becomes evident in the synergic result of the environmental, nutritional and genetic conditions. Material and methods. The HLA phenotypes of the patients were determined by peripheral blood. The product amplification of PCR has been done using the Sequence Specific Primers method (SSP). For the visualisation was carried out an electrophoresis of agarose gel of 2% with an ethidium bromide staining. Results. The HLA alleles type I and II were analysed through a SNP (single-nucleotide polymorphism) method that has a 95% of effectiveness, within an N of 30. It was found that the HLA antigens more frequent were: A*01010101, B*14020104, B*510101-03/05 y DRβ1*04010101. Conclusions. The frequent HLA alleles, as the DRβ1*04010101 and the B*14020104, are directly related to the development of RA and other arthropathies, so having their molecular determination is helpful as an auxiliary for the diagnosis and also contributes to the phylogenetic understanding of the disorder, as well as their relation in the population.

REFERENCES

1. Lugo ZG. El paciente reumático. México: Ed. Europa Press; 2009: 29-31.

2. Feldmann M. Patogenesis of artritis: recent research progress. Nature Immunology 2001; 2(9): 771-3.

3. Firestein GS. Evoluing concepts of rheumatoid arthritis. Nature 2003; 423: 356-61.

4. Mc Carty Daniel. Artritis. México: Editorial Panamericana; 1987: 32.

5. Ruíz RG. Fundamentos de la interpretación clínica de los exámenes de laboratorio. Ed. Panamericana; 2002, p. 139, 342, 372.

6. Rodey GE. Introduction to human histocompatibility. 2nd Ed. USA: Ed. De Novo; 2000:13-37.

7. Bellanti A. Inmunología. 3a Ed. México: Ed. Intercontinental; 1986, p. 62-7.

8. Reguerillo R, López C, González S, Martínez E. Inmunología: Biología y Patología del sistema inmune. México: Ed. Médica Panamericana; 2002, p. 45-53, 65-72.

9. Cunningham A. The structure and function of histocompatibility antigens. Science 1991; (2): 1555-61.

10. Opelz G. HLA Analysis of kidney transplants performed in member countries of mesot. Tran Proc 1997; 29: 2917-19.

11. Garavito G, Iglesias A, Egea E, Jaraquemada D, Martínez P. Una aproximación al significado biológico del polimorfismo del complejo mayor de histocompatibilidad: El modelo de la asociación HLA y ARJ. Salud Uninorte. Barranquilla; 2002; 16: 53-72.

12. Falfan VR. Polimorfismos genéticos en autoinmunidad. Rev Inst Nal Enf Resp Mex 2004; 17(2): 126-34.

13. López de Castro J. HLA-B27 y patogenia de las espondiloartropatías. En la Génesis 2007; 3: 117-19.

14. Delgado V, Granados J, Anaya J, et al. Epidemiología genética de la artritis reumatoide: ¿Qué esperar de América Latina? Biomédica 2006; 26(4): 562-84.

15. Yamamoto K, Granados J. El complejo mayor de histocompatibilidad (MHC) y las enfermedades autoinmunes. Rev Colomb Reumat 1997; 4(1): 19-25.

16. Bunce M, O‘Neill CM, Barnedo, Krause P, Browning MJ, Mornis PJ, Welsh K. Phototyping: Cromprehesive DNA typing for HLA A, B, C, DRB1, DRB3, DRB4, DRB5 and DQB1 by PCR with 144 primers mixes utilising sequence-specific primers (PCR-SSP). Tissue Antigens 1995; 46(51): 355-67.

17. Wodrow J, Nichol F, Zaphiroupoulos G. Antigens and rheumatoid arthritis populations. Br Med J 1981; 238: 1287-88.

18. Domínguez C, Lorenzo N, Barbera A, Hernández V, Torres N, Nazabal M, Camacho M, et al. Caracterización de moléculas HLA II evaluación de citocinas en pacientes cubanos con AR. Rev Cub Reumatología 2006; VIII: 9-10.

19. Sette A, Sidney J. Nine major HLA class I supertypes account for the vast preponderance of HLA-A and -B polymorphism. Immunogenetics 1999; 50: 201.

20. Feldmann M. Patogenesis of arthritis. Recent Research Progress. Nature Immunology 2001; 2(9): 771-73.

21. Rodríguez C, Granados J, Vargas G, Cardie M. Asociación diferencial de los alelos HLA DRB1*0404 y TNF-308*2 en AR grave. Rev Mex Reumat 2004; 19: 11.