Pérez CJC, Gallegos SSP, Garduño PP, Reyes SG, Valderrama GMR, Herrera VI, Arteaga MSM, Herrera SP, Delgado RL

Language: Spanish
References: 64
Page: 99-108
PDF size: 533.62 Kb.

ABSTRACT

Objective. To create a three dimensional (3D) plan model of dissections of medial part of the brain. To get this model we will establish the concentration of formaldehyde, freezing degree and dissection instrumental that offers the best result in dissections of structure of internal brain. Material and methods. Fifteen brains were obtained; blood vessels, duramater and arachnoids meninges were retired, they were divided for their study in four groups. In the three first groups we worked with formaldehyde concentrations, freezing or no freezing degree, and dissection instrumental. In the fourth group, the cerebral hemispheres were immerse en 5% formol fixation, during four weeks; freezed under 15^°C for eight days. Afterwards, topographic dissection in the medial part of the brains with wooden spoonbills, microdissections instrumental and suctions system was carried out; after plans dissections, we carried out a photographic technique for virtual reality and we created a software for 3D presentation of the structures. Results. The immersion of 5% of formol produced less compacting of the gray and white substances than the one immerse at 10%. The freezing at 40^°C does not produce fragmentation of the gray substance matter separation of the white substance. The dissection with wooden spoonbills, microdissection instrumental and suctions system cannulas, allowed a better dissections of the grey and white substance of the brain. We then created and interface between a 3D model of the cerebral structures in anaglyph in each plan of dissections. Conclusion. The fixations of formol at 5%, freezing at 15^°C, and the use of wooden spoonbills, microdissection instru mental and suctions systems, allows the dissection of tracts of 2 mm thickness as the subcallosum tract appears.

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