Tinoco CVC, Quesada CJA, Maldonado RMA, Parra RO, Luna GBA

Language: Spanish
References: 18
Page: 161-165
PDF size: 155.47 Kb.

ABSTRACT

Objective: The aim of the present study was to assess the extent of propolis’ in vitro cytotoxicity on polymorphonuclear leukocytes. Materials and methods: The present study was an in vitro, controlled, experimental endeavor. Statistical procedure entailed variance analysis of repeated measures as well as Scheffe’s post-hoc. A p ‹ 0.05 statistical significance was established. To obtain leukocytes, 10 mL of peripheral venous blood was harvested from six randomly selected, healthy, 20-30 year old subjects, of both genders. Results: Scheffe’s analysis with 95% reliability for comparison between control and experimental groups. Significant with p 0.0001 for propolis 1 control 1 and propolis 2 control 2. For propolis 1 propolis 2 p 0.5002/control 1 control 2 p 0.9621. Conclusions: In the present experiment propolis at a 1:4 dilution applied for 1-2 hours to polymorphonuclear leukocytes caused 70% cellular death. This resulted in statistical significance.

REFERENCES

1. Young K, Matías S, Lima C. Comparação das características fisico químicas das própolis producidas na região sub-tropical da América do Sul; evidencia firoquímica de sua origem botánica. Mesagem Doce. 2001; 61

2. Yong Kun Park, Ikegaki M, de Alencar SM. Clasificação das própolis brasileira a partir de suas características físico-químicas e propriedades biológicas. Mesagem Doce 2000; 58.

3. Bankova V, Christov R, Hegazi A.G, ABD El Hady, Popov S. Chemical composition of propolis from popular budws. International Simposium on Aphiterapy Cairo 1997; 8-9.

4. Dos Santos A, Mendes da Silva JF, Kiltzke R, Nobrega J, de Aquino Neto FR. Identificação do esteres graxos de triterpenoides pentaciclicos em própolis. Mesagem Doce 54.

5. Almas K, Mahmoud A, Dahlan A. A comparative study of propolis and saline applications on human dentin. A SEM study. Indian J Dent Res. 2001; 12 (1): 217.

6. Anaya JM y cols. Inmunología (de memoria). Cap. 4, p. 39, 15a Ed. Corporación para investigaciones biológicas 2009. México, D.F.

7. Guyton A. Fisiología y fisiopatología básicas. 4a ed. Ed. Interamericana 1980. Barcelona, España. Pte. II Células de la sangre, inmunidad y coagulación.

8. Edwards SW. Biochemistry and phisiology of the neutrophil. Cambridgen University Press 1994. New York.

9. Brobeck JR, Best R. Bases fisiológicas de la práctica médica. 10a ed. Ed. Panamericana, República de Argentina. 2006.

10. Jenkins N. Fisiología y bioquímica bucal. 1a ed. Ed. Limusa México 1983: 179-212.

11. Lara M y cols. Estudio in vitro de la actividad antibacteriana del propóleo contra microorganismos de la placa dentobacteriana supragingival. Tesis 2001 Fac de Odontología UAT.

12. Katzung G. Farmacología básica y clínica. Ed. Manual Moderno. México. 1984.

13. Isla M, Nieva, Moreno M, Samprieto A, Vattuone M. Antioxidant activity of Argentine propolis extract. J Ethopharmacoloc. 2001; 76 (2): 165-170.

14. Arce MA y cols. Efecto de las tetraciclinas sobre la quimiotaxis leucocitaria, estudio doble ciego en pacientes con acné vulgaris. Tesis 1983. Monterrey N.L.

15. Rivera y cols. Evaluación de la fagocitosis y digestión intracelular por leucocitos polimorfonucleares de individuos sanos para dos especies de estafilococos coagulasa negativo. Tesis para obtener grado de maestría. UANL Fac de Medicina. 1990 Monterrey N.L.

16. Koo H, Rosalen P et al. Effects of apis mellifera propolis from two brazilian regions on caries development in desalivated rats. Caries Res. 1999; 33 (5): 393-400.

17. Ramírez ME, Villalobos DE, Villafuerte GA, Andrade FF. Propóleo: ¿Una alternativa en la terapéutica médica y odontológica? Primera parte Med Oral. 2001; 2: 91-99.

18. Magro O, Carvalho A. Application of propolis to dental sockets and skin wound. J Nihon Univ Sch Dental. 1999; 36 (2): 4-10.