2013, Number 2
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Rev Cubana Plant Med 2013; 18 (2)
Action of extracts of four plants against infective larvae of gastrointestinal nematodes of sheep
Cabral HI, Rita VC, Gosmann G, Girardi DAM
Language: Portugués
References: 12
Page: 278-287
PDF size: 87.98 Kb.
ABSTRACT
Introduction: an assessment was made of the action of hydroalcoholic extracts of
Eugenia uniflora L. (pitangueira),
Mentha x piperita (menta),
Myrcianthes pungens (O. Berg) D. Legrand (guabiju) and
Peltophorum dubium (Spreng.) Taub. (cañafistula) on the development of gastrointestinal nematodes in fecal cultures from sheep.
Objective: evaluate the
in vitro effect of the plants upon gastrointestinal nematodes of sheep.
Methods: Each extract was tested in triplicate cultures of the faeces at the following concentrations: 200 mg.mL
-1, 100 mg.mL
-1, 20 mg.mL
-1 and 2 mg.mL
-1. moxidectin (0.001 mg.mL
-1) was used as positive control, and distilled water as negative control. After incubation, the larvae were recovered, fixed and stained, and preserved for future reading.
Results: the 200 mg.mL
-1 concentration of
Eugenia uniflora extract was promoted to a decrease in the number of infective larvae, and was recovered from cultures of sheep faeces (number of larvae per gram of faeces = 19), in comparison with other concentrations. This value is 10 times smaller than the one recovered from coprocultures treated with the lowest concentration in the crude extract (2 mg.mL
-1).
Conclusion: statistically, the action of the extract of
Eugenia uniflora at the highest concentration did not differ from the positive control (p< 0.05). This is suggestive of an effect on the culture of gastrointestinal nematodes.
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