Hernández-Jiménez C, Jasso-Victoria R, Olmos-Zúñiga R, Villalba-Caloca J, Gaxiola-Gaxiola M, Sotres-Vega A, Baltazares-Lipp M, Villa-Godoy A

Language: Spanish
References: 27
Page: 222-230
PDF size: 231.66 Kb.

ABSTRACT

Introduction. There are several experimental model of acute lung injury induced by oleic acid (OA); however, there are few studies that show how this injury develops. Objective. This study seeks to detail the x-ray, hemodynamic, gasometrical, gravimetrical, macroscopic and microscopic alterations developed in an experimental model of canine OA-induced acute lung injury (ALI). Material and methods.Twelve dogs were divided in 2 study groups: Group I (n = 6): Control group without ALI. Group II (n = 6); OA-induced ALI. All dogs were submited to X-ray, hemodynamic and gasometric evaluation before ALI induction, and later every 15 minutes during 150 minutes. At the end of the study, the animals were euthanatized and were evaluated the changes gravimetric, macroscopic and microscopic in injured lungs. Results. All the animals survived through the study. In group II, 100% of the animals developed x-ray (p ‹ 0.003 Wilcoxon), hemodynamic, gasometrical and gravimetric (p ‹ 0.5 ANOVA, Tukey), macroscopically and microscopically (p ‹ 0.001 Wilcoxon) ALI. Conclusions. The OA-induced ALI is a model in which dogs develop X-ray, hemodynamic, gasometrical, gravimetrical, macroscopically and microscopically injuries of the exudative phase that lung with ALI injury presents.

REFERENCES

1. Schuster DP, ARDS. Clinical lessons from the oleic acid model of acute lung injury. Am J Resp Crit Care Med, 1994; 149: 245-60.

2. Gordon D, Rubenfeld MD, Ellen Caldwell MS. Incidence and outcomes of acute lung injury. N Engl J Med 2000; 342: 1301-8

3. Matute-Bello G, Frevert WC, Martin TR. Animals models of acute lung injury. Am J Physiol Lung Cell Mol Physiol 2008; 295: L379-L399.

4. Beilman G. Pathogenesis of oleic-induced lung injury in the rat: distribution of oleic acid during injury and early endothelial cell changes. Lipids 1995; 30: 817-23.

5. Ashbaugh DG, Uzawa T. Respiratory and hemodynamic changes after injection of free fatty acids. J Surg Res 1968; 8: 417-23.

6. Especificaciones técnicas para la producción, cuidado y uso de animales de laboratorio. Diario Oficial de la Federación de los Estados Unidos mexicanos. AFIA 6 Dic de 1999.

7. National Institutes of Health U.S.A. Guía para el cuidado y uso de los animales de laboratorio. Department of Health and Human Services, Public Health Service, National Institutes of Health U.S.A. México, Edición Mexicana Auspiciada por la Academia Nacional de Medicina; 2002.

8. Bernard GR, Artigas A, Brigham KL, Carlet J, Hudson L, et al The American–European consensus conference on ARDS: definitions, mechanism, relevant outcomes and clinical coordination. Am J Resp Crit Care Med 1994; 149: 818-24.

9. Schoene R, Robertson H, Thorning D, Springmeyer S. Pathophysiological patterns of resolution from acute oleic acid injury in the dog. J Appl Physiol 1984; 56: 472-81.

10. Miyazawa T, Nakagawa H, Hiramoto M, Arita K, Hiramoto T, Nishida O. Ultrastructural study on the alveolar-capillary injury with pulmonary edema induced by oleic acid in dogs. Hiroshima J Med Sci 1981; 30: 183-90.

11. Trawöger R, Cereda M, Kolobow T. A standardized method of oleic acid infusion in experimental acute respiratory failure Scan. J Clin Lab Invest 2001; 61: 75-82.

12. Wang HM, Bodenstein M, Markstaller K. Overview of the pathology of three widely used animal’s models of acute lung Injury. Eur Surg Res 2008; 40: 305-16.

13. Zwissler B, Forst H, Ishii K, Messmer K. A new experimental model of ARDS and pulmonary hypertension in the dog. Res Exp Med 1989; 189: 427-38.

14. Derks CM, Jacobovitz-Derks D. Embolic pneumopathy induced by oleic acid a systemic morphologic study. Am J Pathol 1977: 87; 143-58.

15. Grotjohan HP, van der Heijde RM, Jansen JR, Wagenvoort CA, Versprill A. A stable model of respiratory distress by small injections of oleic acid in pigs. Intensive Care Med 1996; 22: 336-44.

16. Scillia P, Abdel KS, Mélot C, Keyzer C, et al. Oleic acid-induced lung injury: Thin section CT evaluation in dogs. Radiology 2001; 219: 724-31.

17. Zhou Zhaohui, Kozlowski J, Schuster P. Physiologic, biochemical, and imaging characterization of acute lung injury in mice. Am J Resp Crit Care Med 2005; 172: 344-55.

18. Pagnamenta A, Bouckaefert Y, Wauthy P. Continuous versus pulsatile pulmonary hemodynamics in canine oleic acid lung injury. Am J Respir Crit Care Med 2000; 162(3): 936-40.

19. Kafi SA, Scillia P, Melot C, et al. Abnormal pulmonary vascular tone in canine oleic acid lung injury. Crit Care Med 2002; 30: 1565-9.

20. Leeman M, Lejeune P, Clozel J, Cachiery JL, Melot C, Naeijie R. Nature of pulmonary hypertension in canine oleic acid pulmonary edema. J Apply Physiol 1990; 69: 293-8.

21. Putensen C, Räsänen J, Downs BJ. Effect of endogenous and inhaled nitric oxide on the ventilation-perfusion relationships in oleic. Acid lung injury. Am J Crit Care Med 1994: 150; 330-6.

22. Cristancho W. Circulación pulmonar y relación ventilación perfusión. En Cristancho W. Fisiología respiratoria. Lo esencial en la práctica clínica. 1ra ed. Bogota Colombia. Manual Moderno. 2004; p. 57-81.

23. Vadasz I, Morty RE, Kohstall MG, Olschewski A, Grimminger F, Seeger W. Oleic acid inhibits alveolar fluid reabsortion: A role in acute lung respiratory distress syndrome? Am J Resp Crit Care Med 2005; 171: 469-79.

24. Eiermann G, Dickey B, Thrall R: Polymorphonuclear leukocyte participation in acute oleic-acid-induced lung injury. Am Rev Resp Dis 1983; 128: 845-50.

25. Ito K, Mizutani A, Kira S, Mori M, Iwasaka H, Noguchi T. Effect of ulinastatin, a human urinary trypsin inhibitor, on the oleic acid induced acute lung injury in rats via the inhibition of actived leukocytes. Injury 2005; 36: 387-94.

26. Hoeffel JM, Flick M. Oleic acid injury in sheep. J Appl Physiol 1986; 60: 433-40.

27. Hofman W, Ehrhart I. Permeability edema in dog lung depleted of blood components. J Appl Physiol 1984; 57: 147-53.