Villagrana ZJR, López HM, Flores SVR, de Haro CMJ, Escobedo GMR, Guerra IFM

Language: Spanish
References: 30
Page: 23-28
PDF size: 241.61 Kb.

ABSTRACT

Background: Chlamydia trachomatis infection is considered a public health problem due to its high prevalence, and because is asymptomatic in 70% of women and provokes reproductive sequelae when it is not detected and treated timely.
Objective: To search for C. trachomatis in endometrium and peritoneal fluid of infertile women without detection of this pathogen in cervical secretions.
Patients and method: A retrospective and cross-sectional study was done in 38 patients with infertility only 18 showed peritoneal fluid infection and/or endometrial infection, eight of them were negative for the amplificated product of 129-bp from CT ompA gene in cervical secretions. Laparoscopic data showed that five of them had pelvic inflammatory disease.
Conclusion: The non-detection of Chlamydia trachomatis in endocervix does not reflect what happens in the upper genital tract, that’s why we need to do a deliberate search of infection by this pathogen in endometrium of suspected women with infertility.

REFERENCES

1. Haggerty CL, Gottlieb SL, Taylor BD, Low N, et al. Risk of sequelae after Chlamydia trachomatis genital infection in women. J Infect Dis 2010;201(Suppl 2):S134-155.

2. Bakken IJ. Chlamydia trachomatis and ectopic pregnancy: recent epidemiological findings. Curr Opin Infect Dis 2008;21(1):77-82.

3. Ostergaard L, Birkelund S, Christiansen G. Use of polymerase chain reaction for detection of Chlamydia trachomatis. J Clin Microbiol 1990;28(6):1254-1260.

4. Bass CA, Jungkind DL, Silverman NS, Bondi JM. Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 1993;31(10):2648-2653.

5. Wølner-Hanssen P. Silent pelvic inflammatory disease: is it overstated? Obstet Gynecol 1995;86(3):321-325.

6. Oakeshott P, Hay P. General practice update: Chlamydia infection in women. Br J Gen Pract 1995;45(400):615-620.

7. Haggerty CL, Gottlieb SL, Taylor BD, Low N, et al. Risk of sequelae after Chlamydia trachomatis genital infection in women. J Infect Dis 2010;201(Suppl 2):S134-S155.

8. Toth M, Patton DL, Campbell LA, Carretta EI, et al. Detection of chlamydial antigenic material in ovarian, prostatic, ectopic pregnancy and semen samples of culture-negative subjects. Am J Reprod Immunol 2000;43(4):218-222.

9. Patton DL, Askienazy-Elbhar M, Henry-Suchet J, Campbell LA, et al. Detection of Chlamydia trachomatis in fallopian tube tissue in women with postinfectious tubal infertility. Am J Obstet Gynecol 1994;171(1):95-101.

10. Broeze KA, Opmeer BC, Coppus SF, Van Geloven N, et al. Chlamydia antibody testing and diagnosing tubal pathology in subfertile women: an individual patient data meta-analysis. Hum Reprod Update 2011;17(3):301-310.

11. Hernández-Trejo M, López-Hurtado M, Arteaga-Troncoso G, Guerra-Infante FM. Evaluación de los niveles séricos de anticuerpos IgG anti-Chlamydia trachomatis para el diagnóstico de infertilidad por factor tubario. Rev Invest Clin 2009;61(1):87-89.

12. Sharara FI, Queenan JT Jr, Springer RS, Marut EL, et al. Elevated serum Chlamydia trachomatis IgG antibodies. What do they mean for IVF pregnancy rates and loss? J Reprod Med 1997;42(5):281-286.

13. Black CM. Current methods of laboratory diagnosis of Chlamydia trachomatis infections. Clin Microbiol Rev 1997;10(1):160-184.

14. Jiménez-Estrada JM, Escobedo-Guerra MR, Arteaga-Troncoso G, López-Hurtado M, et al. Detection of Chlamydophila abortus in sheep (Ovies aries) in Mexico. Amer J Anim Vet Scien 2008;3(4):91-95.

15. Dutilh B, Bébéar C, Rodriguez P, Vekris A, et al. Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction. Res Microbiol 1989;140(1):7-16.

16. Guerra-Infante FM, Flores-Medina S, López-Hurtado M, Zamora-Ruiz A, et al. Tumor necrosis factor in peritoneal fluid from asymptomatic infertile women. Arch Med Res 1999;30(2):138-143.

17. Bell JD, Bergin IL, Schmidt K, Zochowski MK, et al. Nonhuman primate models used to study pelvic inflammatory disease caused by Chlamydia trachomatis. Infect Dis Obstet Gynecol 2011; doi:10.1155/2011/675360.

18. Bell JD, Bergin IL, Harris LH, Chai D, et al. The effects of a single cervical inoculation of Chlamydia trachomatis on the female reproductive tract of the baboon (Papio anubis). J Infect Dis 2011;204(9):1305-1312.

19. Wolner-Hanssen WH, Mardh PA. In vitro tests of the adherence of Chlamydia trachomatis to human spermatozoa. Fertil Steril 1984;42:102-107.

20. Friberg J, Confino E, Suarez M, Gleicher N. Chlamydia trachomatis attached to spermatozoa recovered from the peritoneal cavity of patients with salpingitis. J Reprod Med 1987;32(2):120-122.

21. Zervomanolakis I, Ott H, Hadziomerovic D, Mattle V, et al. Physiology of upward transport in the human female genital tract. Ann N Y Acad Sci 2007;1101:1-20.

22. Taylor BD, Haggerty CL. Management of Chlamydia trachomatis genital tract infection: screening and treatment challenges. Infect Drug Resist 2011;4:19-29.

23. Narcio RML, Arredondo GJL, Zaldívar NA, Quesnel GBC y col. Etiología microbiana de la enfermedad inflamatoria pélvica leve y moderada (EIP). Ginecol Obstet Mex 1998;66:309-315.

24. Sweet RL. Treatment of acute pelvic inflammatory disease. Infect Dis Obstet Gynecol 2011; Article ID 561909, 13 pages, doi:10.1155/2011/561909.

25. Oakeshott P, Kerry S, Aghaizu A, Atherton H, et al. Randomised controlled trial of screening for Chlamydia trachomatis to prevent pelvic inflammatory disease: the POPI (prevention of pelvic infection) trial. BMJ 2010;340:c1642. doi:10.1136/ bmj.c1642.

26. Ness RB, Smith KJ, Chang CC, Schisterman EF, Bass DC. Gynecologic infection follow-through, GIFT, investigators. Prediction of pelvic inflammatory disease among young, single, sexually active women. Sex Transm Dis 2006;33(3):137-142.

27. Haggerty CL, Ness RB, Amortegui A, Hendrix SL, et al. Endometritis does not predict reproductive morbidity after pelvic inflammatory disease. Am J Obstet Gynecol 2003;188(1):141-148.

28. Dieterle S, Wollenhaupt J. Humoral immune response to the chlamydial heat shock proteins hsp60 and hsp70 in Chlamydiaassociated chronic salpingitis with tubal occlusion. Hum Reprod 1996;11(6):1352-1356.

29. Toye B, Laferrière C, Claman P, Jessamine P, Peeling R. Association between antibody to the chlamydial heat-shock protein and tubal infertility. J Infect Dis 1993;168(5):1236-1240.

30. Mascellino MT, Boccia P, Oliva A. Immunopathogenesis in Chlamydia trachomatis infected women. Obstet Gynecol 2011;2011:436936. (En prensa.)