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ISSN 2992-8559 (Electronic)
ISSN 1405-0625 (Print)

2012, Number 1

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Cir Plast 2012; 22 (1)

Characterization of acellular homologous bone as tissue engineering scaffold

Erazo CCA, Molina MF

Language: Spanish
References: 11
Page: 6-10
PDF size: 236.76 Kb.


ABSTRACT

Tissue regeneration by tissue engineering requires scaffolds for culture cells to reproduce the in vivo three dimensional arrays restoring form and function. This work aims to characterize the acelular homologous bone as a new biological scaffold for bone tissue engineering. The homologous bone is stripped from its cells and proteins by humid steam, leaving the mineral matrix. To prove that no viable cell is left in the scaffold we designed an experimental study in six New Zealand adult rabbits. Two samples of trabecular iliac crest bone were taken and one of them was exposed to humid steam according to our protocol. Cellular cultures from both samples were followed for six weeks. No cellular growth at six weeks was observed in the humid steam group compared to the full cellular growth in the control group since week one. We prove that homologous bone treated by humid steam according to our protocol is acellular. On the other hand, there seems to be small quantities of residual collagen, identified by protein electrophoresis in our scaffold.


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