2012, Number 1
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Rev Med Inst Mex Seguro Soc 2012; 50 (1)
Systemic steroids use in second degree burn using an animal model
Ramos-Gallardo G, Ambriz-Plascencia AR, González-Reynoso L
Language: Spanish
References: 13
Page: 9-12
PDF size: 48.24 Kb.
ABSTRACT
Background: the use of steroids is recognized in septic shock.
There are reports of their use in burns. It is also known their
negative effect in wound healing. Objective: to know the effect
of steroids in burn healing.
Methods: two groups of ten rats (wistar) were exposed to metallic
cylinder at 95 °C for 15 seconds on the back. At the moment of
the burn one group received hydrocortisone dose 5 mg/kg. The
other group didn’t received medication. The scar was removed at
the fifth day and the burn injury was covered with queratinocyte
culture. The rats were sacrificed at 14th day. The presence of
infection and the percentage of new epithelium, fibrosis, inflammatory
process, presence of fibroblast and vascular proliferation
were evaluated. We compared both groups using χ2 test.
Results: there are no difference between groups in fibrosis, inflammatory
process, or fibroblast presence. But there is a difference
in vascular proliferation against the first group (steroid
group) There were no signs of infection and all of them were
epithelized at the 14th day.
Conclusions: the use of steroids in burns only showed difference
in vascular proliferation.
REFERENCES
Surviving Sepsis Campaign International. Guidelines for management of severe sepsis and septic shock. Crit Care Med 2008;36(1):296-327.
Sligl WI, Milner DA, Sundar S, Mphatswe W, Majumdar SR. Safety and efficacy of corticosteroids for the treatment of septic shock: a systematic review and meta-analysis. Clin Infect Dis 2009;49(1):93-101. Disponible en http:/ /cid.oxfordjournals.org/content/49/1/93.long
Annane D, Bellissant E, Bollaert PE, Briegel J, Confalonieri M, De Gaudio R, et al. Corticosteroids in the treatment of severe sepsis and septic shock in adults: a systematic review. JAMA 2009;301(22):2362-2375.
Sprung CL, Goodman S, Weiss YG. Steroid therapy of septic shock. Critical Care Clin 2009;25(4):825-834.
Groeneveld AB, Molenaar N, Beishuizen B. Should we abandon corticosteroids during septic shock? No. Curr Opin Crit Care 2008;14(4):384-389.
Fuchs PCh, Bozkurt A, Johnen D, Smeets R, Groger A, Pallua N. Beneficial effect of corticosteroids in catecholamine- dependent septic burn patients. Burns 2007;33(3): 306-311.
Winter W, Kamolz L, Donner A, Hoerauf K, Blaicher A, Andel H. Hydrocortisone improved haemodynamics and fluid requirements in surviving but not non-surviving of severely burned patients. Burns 2003;29(7):717-720.
Wong J. Laboratory animal care policies and regulations: Canada. ILAR J 1995;37(2):57-59.
Sun W, Watanabe Y, Toki A, Wang ZQ. Beneficial effects of hydrocortisone in induced acute pancreatitis or rats. Chin Med J 2007;120(20):1757-1761.
Pham C, Greenwood J, Cleland H, Woodruff P, Madden G. Bioengineered skin substitutes for the management of burns: a systematic review. Burns 2007;33(8):946-957.
Broughton G 2nd, Janis JE, Attinger CE. Wound healing: an overview. Plast Reconstr Surg 2006;117(7 Suppl);1e-S- 32e-S.
Hunt JL, Purdue GF, Zbar RIS. Burns acute burns, burn surgery and postburn reconstruction. En: Selected Readings in Plastic Surgery. Dallas, TX; Selected Readings in Plastic Surgery; 2000, volume 9, number 12.
Fuchs PCh, Groger A, Bozkurt A, Johnen D, Wolter T, Pallua N. Cortisol in severely burned patients: investigations on disturbance of the hypothalamic-pituitaryadrenal axis. Shock 2007;28(6):662-667.
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