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ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2012, Number 3

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Biotecnol Apl 2012; 29 (3)

Evidences of autochthonous infection by Borrelia burgdorferi sensu lato in Cuba

Rodríguez I, Fernández C, Fuentes O, González RA, Martínez B, Echevarría E, Noda AA, Obregón AM

Language: English
References: 17
Page: 190-193
PDF size: 110.97 Kb.


ABSTRACT

Borrelia burgdorferi sensu lato is the causative agent of Lyme disease, borreliosis not reported in Cuba but clinical and epidemiological suspicions exist since the 1980’s, and there were no microbiological testing for confirmation. This led to perform a series of researches to provide scientific evidences about the presence of this agent in the country, including the evaluation and implementation of microbiological methods for the detection of this spirochete, confirmation of infection in clinical samples from patients with clinical and epidemiological suspicions, the estimated seroprevalence of antibodies against this agent in a population at risk and the molecular detection of borrelia in ticks of veterinary and medical importance. The microbiological tools evaluated (culture medium modified, methods of extracting genetic material from ticks for subsequent detection of B. burgdorferi sensu lato by molecular testing, and analytical sensitivity of two sets of primers) and implemented (specific serological tests) strengthened the IPK analytical capacity with novel methods for future researches. We found specific serological evidences of autochthonous infection by this organism in samples of individuals with clinical and epidemiological suspicion, and those exposed to tick bites. Genetic material of B. burgdorferi sensu lato was undetected in the analyzed ticks, what does not rule them out as potential carriers of the agent. In this paper we show evidence highly suggestive of infection with B. burgdorferi sensu lato and it is the first of its kind in the country.


REFERENCES

  1. Stanek G, Reiter M. The expanding Lyme Borrelia complex--clinical significance of genomic species? Clin Microbiol Infect. 2011;17(4):487-93.

  2. Gern L. Tiques et borréliose de Lyme en Suisse occidentale. Bull Soc Neuchateloise Scie Nat. 2004;127(1):5-21.

  3. Steere AC. Lyme disease. N Engl J Med. 2001;345(2):115-25.

  4. Grandío O, Fernández A, Fernández M, Valera R, Fuentes O, Pelegrino J. Informe preliminar sobre investigaciones realizadas en el poblado “Las Terrazas”, Sierra del Rosario, sobre la existencia de la enfermedad de Lyme. Rev Cubana Pediatr. 1988;60(5):773.

  5. Dickinson F, Fuentes O, Fernández A. ¿Borreliosis de Lyme en Cuba? In: Comité Organizador, editores. Abstracts of the V Congreso Latinoamericano de Medicina Tropical; 1997 Mar 3-7. La Habana: Instituto de Medicina Tropical Pedro Kourí. p. 172-3.

  6. Barbour AG. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984;57(4):521-5.

  7. Ruzic-Sabljic E, Strle F. Comparison of growth of Borrelia afzelii, B. garinii, and B. burgdorferi sensu stricto in MKP and BSK-II medium. Int J Med Microbiol. 2004;294(6):407-12.

  8. Wen B, Jian R, Zhang Y, Chen R. Simultaneous detection of Anaplasma marginale and a new Ehrlichia species closely related to Ehrlichia chaffeensis by sequence analyses of 16S ribosomal DNA in Boophilus microplus ticks from Tibet. J Clin Microbiol. 2002;40(9):3286-90.

  9. Halos L, Jamal T, Vial L, Maillard R, Suau A, Le Menach A, et al. Determination of an efficient and reliable method for DNA extraction from ticks. Vet Res. 2004;35(6):709-13.

  10. Fraga J, Rodríguez J, Fuentes O, Castex M, Fernández-Calienes A. Comparación entre 5 métodos para la extracción de ADN de triatomíneos: su utilización en la técnica de ADN polimórfico amplificado al azar. Rev Cubana Med Trop. 2004;56(3):208-13.

  11. Gaillard C, Strauss F. Ethanol precipitation of DNA with linear polyacrylamide as carrier. Nucleic Acids Res. 1990;18(2):378.

  12. Humair PF, Douet V, Moran Cadenas F, Schouls LM, Van De Pol I, Gern L. Molecular identification of bloodmeal source in Ixodes ricinus ticks using 12S rDNA as a genetic marker. J Med Entomol. 2007;44(5):869-80.

  13. Postic D, Assous MV, Grimont PA, Baranton G. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol. 1994;44(4):743-52.

  14. Marconi RT, Garon CF. Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J Clin Microbiol. 1992;30(11):2830-4.

  15. Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease. MMWR Morb Mortal Wkly Rep. 1995;44(31):590-1.

  16. Hilton E, Devoti J, Sood S. Recommendation to include OspA and OspB in the new immunoblotting criteria for serodiagnosis of Lyme disease. J Clin Microbiol. 1996;34(6):1353-4.

  17. Poupon MA, Lommano E, Humair PF, Douet V, Rais O, Schaad M, et al. Prevalence of Borrelia burgdorferi sensu lato in ticks collected from migratory birds in Switzerland. Appl Environ Microbiol. 2006;72(1):976-9.




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