2012, Number 4
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Rev Cubana Plant Med 2012; 17 (4)
Phytochemical screening and antibacterial activity of dry extracts of 20 % tinctures from Mammea americana L.
Remón RH, Alarcón ZA, Almeida SM, Viera TY, Ramos EM, Bazan OY
Language: Spanish
References: 21
Page: 300-307
PDF size: 71.89 Kb.
ABSTRACT
Introduction: the yellow mammee (Mammea americana L.) is widely used to cure different diseases caused by parasites and insects.
Objective: to perform a phytochemical screening and to determine antibacterial activity of dry extracts of 20 % tincture from the leaves and the bark of this plant.
Methods: the leaves and stem bark of the plant were harvested, washed, disinfected, dried and crushed. Then it proceeded to the preparation of 20 % tinctures through drug maceration. The dried extracts of the studied parts were obtained from the 20 % tinctures and used to evaluate the phytochemical screening tests and the antibacterial activity.
Results: the existence of a high diversity of secondary metabolites, predominantly alkaloids, coumarins, phenols and / or tannins, quinones, and flavonoids were confirmed. The dried extracts showed antibacterial activity in vitro against Staphylococcus aureus (ATCC 29737).
Conclusions: flavonoids, alkaloids, tannins and coumarins, which are of biological and pharmacological interest for their potential therapeutic applications, were significantly present in these extracts.
REFERENCES
Las plantas medicinales. Primera parte. Trabajo original en el Boletín de Nutrición Infantil CANIA. Año 4, No. 8. noviembre de 2003[Citado 5 Abr 2011]. Disponible en: http://www.slan.org.ve/publicaciones/completas/plantas_medicinales_1.asp
Peña A, Torres E. Algunas consideraciones sobre el empleo de productos naturales en la medicina natural y tradicional. Monografía. Bayamo, Cuba: Universidad de Granma; 2002. p. 2-6.
Rodríguez A. Actividad antifúngica in vitro de una crema de Plantago major L. (Llantén). Rev Cubana Plant Med. 1996;1(3):9-12.
Martínez CM, Victorio FM, Campos MD. Determinación del efecto diurético de Cymbopogon citratus (DC) Staff (caña santa). Rev Cubana Plant Med. 1996;1(3):13-7.
Monte MA. Perspectiva de la fitoterapia. Acta Farm Bonaerense. 1990;9(2):131-9.
León MC. Efecto diurético y toxicidad aguda del Orthosiphon aristatus (Blume) Té de Riñón. Rev Cubana Plant Med. 1996;1(3):26-30.
Bantar C, Sartori B, Vesco E. A hospital wide intervention program to optimize the quality of antibiotic use: impact on prescribing practice, antibiotic consumption, cost savings, and bacterial resistance. Clin Infect Dis 2001; 32: 1162-71.
Jasovich A. El control de los antibióticos ¿Hasta donde duela? Rev Chil Infect. 2003;20(1):63-9.
Cuba. Ministerio de Salud Pública. Norma Ramal de Salud Pública No. 309. Medicamentos de origen vegetal: droga cruda. Métodos de ensayos. La Habana: MINSAP; 1992.
Carballo C. Desinfección química de Pedilanthus tithymaloides L. Poit. Rev Cubana Plant Med. 2005;10(2):10-3.
Cuba. Ministerio de Salud Pública. Norma Ramal de Salud Pública No. 312. Métodos de ensayos para la determinación de los requisitos de los extractos fluidos y tinturas. La Habana: MINSAP; 1998.
Cuba. Ministerio de Salud Pública. Norma Cubana Salud Pública-313. Métodos de ensayos a partir de drogas crudas. Salud Pública. La Habana. MINSAP. 1998.
Cuba. Programa Nacional de Medicina Tradicional y Natural. Ministerio de Salud Pública. La Habana: MINSAP; 1999.
Sandoval D, Suárez O. Estudio fitoquímico preliminar de detección de alcaloides y saponinas en plantas que crecen en Cuba. Rev Cubana Farm. 1990;24(2):288-96.
Payo A. Tamizaje fitoquímico preliminar de plantas que crecen en Sierra de Nipe, Holguín. Rev Cubana Farm. 1996;30(2):120-31.
Clinical and Laboratory Standards Institute. Disk Diffusion Supplemental Tables. M100-S17 (M2). CLSI: Wayne Pa; 2007.
Taiz LT, Zeiger E. Secondary Metabolites and Plant Defense. Plant Physiology. 4th ed. USA: Sinauer Associates, Inc.; 2006.
Gurinder JK, Daljit SA. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complementary Alternative Med. 2009;9:30.
Ahmet A. Antimicrobial activity of the essential oil of Cyclotrichium niveum (Boiss.) Manden. Et Scheng. African J Microbiol Research. 2009;3(8):463-7.
Lambert PA. Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. J Royal Society Med. 2002;95(41):22-6.
Sukanya SL. Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. African J Biotechnology. 2009;8(23):6677-82.