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Órgano oficial de la Facultad de Odontología, UNAM

2013, Number 1

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Rev Odont Mex 2013; 17 (1)

Citotoxicity assessment of different endodontic-use sealing cements in gingival fibroblast cultures

Cortázar FC, García ARL, Willershausen I, Willershausen B, Briseño MB

Language: Spanish
References: 36
Page: 33-41
PDF size: 734.92 Kb.


ABSTRACT

The aim of the present study was the in vitro evaluation of the response, within 96 hours, of gingival fibroblast cultures with respect to different endodontic sealers. Results obtained at time intervals of 0, 1, 2, 3, 6, 24, 48, 72 and 96 hours were used to determine sealers´ cytotoxicity. Gingival fibroblasts cultures without root canal sealer and with Sealapex were used as negative and positive controls respectively. Results were compared with negative controls and statistically analyzed with t Dunnett test (p ≤ 0.05). Assessed sealing cements were: ProRoot MTA, grey and white, CPM, MTA Angelus, Sealapex and GuttaFlow. Results showed that even though ProRoot MTA (grey and white) MTA Angelus, CPM and GuttaFlow exhibited lower cytotoxic potential than Sealapex, no statistical significant differences were established.


REFERENCES

  1. Seltzer S, Sinai I, August D. Periodontal effects of root perforations before and during endodontic procedures. J Dent Res 1970; 49(2): 332-339.

  2. Torabinejad M, Chivian N. Clinical applications of mineral trioxide aggregate. J Endod 1999; 25(3): 197-205.

  3. Lee S, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 1993; 19(11): 541-544.

  4. Shah PM, Chong BS, Sidhu SK, Ford TR. Radiopacity of potential root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1996; 81(4): 476-479.

  5. Torabinejad M, Hong C, McDonald F, Pitt Ford T. Physical and chemical properties of a new root-end filling material. J Endod 1995; 21(7): 349-353.

  6. Campos QI, Llamosas HE, Morales de la Luz R. Evaluación de la biocompatibilidad del cemento Portland implantado en tejido conectivo subepitelial de ratas. Rev ADM 2003; 60(2): 45-51.

  7. García-Aranda RL, García-Garduño MV. Análisis químico por difracción y fluorescencia de rayos X de los cementos Portland, blanco, ProRoot gris y blanco, Angelus blanco y CPM. Endodoncia Actual 2009; 4(2): 6-11.

  8. Dental DT. ProRoot® MTA (mineral trioxide aggregate) root canal repair material. Material safety data. Dentsply Tulsa Dental 2002: 1-2.

  9. Torabinejad M, Smith P, Kettering J, Pitt Ford T. Comparative investigation of marginal adaptation of mineral trioxide aggregate and other commonly used root-end filling materials. J Endod 1995; 21(6): 295-299.

  10. Holland R, de Souza V, Nery MJ, Otoboni Filho JA, Bernabé PF, Dezan JE. Reaction of rat connective tissue to implanted dentin tubes filled with mineral trioxide aggregate or calcium hydroxide. J Endod 1999; 25(3): 161-166.

  11. Baek S, Plenk H, Kim S. Periapical tissue responses and cementum regeneration with amalgam, SuperEBA, and MTA as root-end filling materials. J Endod 2005; 31(6): 444-449.

  12. Thomson T, Berry J, Somerman M, Kirkwood K. Cementoblasts maintain expression of osteocalcin in the presence of mineral trioxide aggregate. J Endod 2003; 29(6): 407-412.

  13. Torabinejad M, Pitt Ford T, McKendry D, Abedi HR, Miller D, Kariyawasam S. Histologic assessment of mineral trioxide aggregate as a root-end filling in monkeys. J Endod 1997; 23(4): 225-228.

  14. Koh ET, Torabinejad M, Pitt Ford TR, Brady K, McDonald F. Mineral trioxide aggregate stimulates a biological response in human osteoblasts. J Biomed Mater Res 1997; 37(3): 432-439.

  15. Pistorius A, Willershausen B, Briseño Marroquin B. Effect of apical root-end filling materials on gingival fibroblasts. Int Endod J 2003; 36(9): 610-615.

  16. Osorio R, Hefti A, Vertucci F, Shawley A. Cytotoxicity of endodontic materials. J Endod 1998; 24(2): 91-96.

  17. Keiser K, Johnson C, Tipton D. Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts. J Endod 2000; 26(5): 288-291.

  18. Camps J, About I. Cytotoxicity testing of endodontic sealers: a new method. J Endod 2003; 29(9): 583-586.

  19. Briseño B, Willershausen B. Root canal sealer cytotoxicity on human gingival fibroblasts. 1. Zinc oxide-eugenol-based sealers. J Endod 1990; 16(8): 383-386.

  20. Briseño BM, Willershausen B. Root canal sealer cytotoxicity on human gingival fibroblasts: 2. Silicone- and resin-based sealers. J Endod 1991; 17(11): 537-540.

  21. Briseño BM, Willershausen B. Root canal sealer cytotoxicity with human gingival Fibroblasts. III. Calcium hydroxide-based sealers. J Endod 1992; 18(3): 110-113.

  22. Willershausen B, Marroquín BB, Schäfer D, Schulze R. Cytotoxicity of root canal filling materials to three different human cell lines. J Endod 2000; 26(12): 703-707.

  23. Torabinejad M, Hong C, Lee S, Monsef M, Pitt Ford T. Investigation of mineral trioxide aggregate for root-end filling in dogs. J Endod 1995; 21(12): 603-608.

  24. Pitt Ford TR, Torabinejad M, McKendry DJ, Hong CU, Kariyawasam SP. Use of mineral trioxide aggregate for repair of furcal perforations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995; 79(6): 756-763.

  25. Torabinejad M, Hong C, Pitt Ford T, Kaiyawasam S. Tissue reaction to implanted super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report. J Endod 1995; 21(11): 569-571.

  26. Torabinejad M, Ford T, Abedi HR, Kariyawasam S, Tang H. Tissue reaction to implanted root-end filling materials in the tibia and mandible of guinea pigs. J Endod 1998; 24(7): 468-471.

  27. Fischer E, Arens D, Miller C. Bacterial leakage of mineral trioxide aggregate as compared with zinc-free amalgam, intermediate restorative material, and Super-EBA as a root-end filling material. J Endod 1998; 24(3): 176-179.

  28. Torabinejad M, Rastegar A, Kettering J, Pitt Ford T. Bacterial leakage of mineral trioxide aggregate as a root-end filling material. J Endod 1995; 21(3): 109-112.

  29. Ford TR, Torabinejad M, Abedi HR, Bakland LK, Kariyawasam SP. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc 1996; 127(10): 1491-1494.

  30. Martin GM. Human skin fibroblasts. 1973: 39-43.

  31. Ian Freshney R. Tierische Zellkulturen: Ein Methoden-Handbuch. 1990: 404.

  32. Dutrillaux B, Couturier J. Praktikum der Chromosomenanalyse. 1983: 77.

  33. Hession RW. Long-term evaluation of endodontic treatment: anatomy, instrumentation, obturation--the endodontic practice triad. Int Endod J 1981; 14(3): 179-184.

  34. Milleding P, Wennberg A, Hasselgren G. Cytotoxicity of corroded and non-corroded dental silver amalgams. Scand J Dent Res 1985; 93(1): 76-83.

  35. Wennberg A, Hasselgren G. Cytotoxicity evaluation of temporary filling materials. Int Endod J 1981; 14(2): 121-124.

  36. Camp M, Jeansonne B, Lallier T. Adhesion of human fibroblasts to root-end-filling materials. J Endod 2003; 29(9): 602-607.




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