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2011, Number 2

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VacciMonitor 2011; 20 (2)

Adjuvant activity of rice oil on the immune response to ovalbumin

Maciel SIT, Moleta CE, Albuquerque D

Language: English
References: 60
Page: 1-5
PDF size: 166.64 Kb.


ABSTRACT

We have evaluated the adjuvant activity of rice oil (RO) on the immune response to ovalbumin (OVA), its depots and possible side effects at the injection sites. Immunization of mice with OVA emulsified in rice oil (OVA+RO) resulted in an antibody response significantly higher than that determined in mice immunized with soluble OVA. In addition, these high antibody levels were observed for a period as long as 6 weeks after immunization. However, the adjuvant action of RO was significantly lower to incomplete Freund’s adjuvant (IFA). In secondary immune response, the pattern of OVA-specific antibodies stimulated by RO was predominantly IgG1. Despite of the significant humoral response enhanced by RO at no time was noted any lesions at the site of injection. Besides, histological analysis showed that inflammatory reaction caused by RO was mild and transient, suggesting that RO appears to be a safe, effective and chemically define alternative to IFA in many situations.


REFERENCES

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  2. Claasen E, Leeuw W, Greve P, Hendriksen C, Boersma W. Freund’s complete adjuvant: an effective but disagreable formula. Res Immunol 1992;143:478-83.

  3. Mbow ML, Gregorio E, Valiante NM, Rappuoli R. New adjuvants for human vaccine, Current Opinion in Immunol 2010;22:411- 6.

  4. Amyx HL. Control of animal pain and distress in antibody production and infectious disease studies. J Am Vet Med Assoc 1987; 191:1287-9.

  5. Lambrecht BN, Kool M, Willart MAM, Hammad H. Mechanism of action of clinically approved adjuvants. Current Opinion in Immunology 2009; 21:23-9.

  6. Reed SG, Bertholet S, Coler RN, Friede M. New horizons in adjuvants for vaccine development. Trends in Immunol 2009;30:23-32.

  7. Moro E, Junqueira JOB. Levantamento da incidência de reações vacinais e/ou medicamentosas em carcaças de bovinos ao abate em frigoríficos no Brasil. A hora Vet 1999;122:74-7.

  8. França AT, Alves GG, Mesquita AJ, Chiquetto CE, Bueno CP, Oliveira ASC. Perdas econômicas por abscessos vacinais e/ ou medicamentosos em carcaças de bovinos abatidos no estado de Goiás. Ciência Animal Brasileira 2006;7(1):93-6.

  9. Hilleman MR, Woodhour AF, Friedman A, Weibel RE, Stokes J. The clinical application of adjuvant-65. Ann Allergy 1972;30:152-8.

  10. Silva ACM, Guerra JL, Melo DF, Lima MS. Adjuvant effect of corn and olive oils in mouse immune response. In: VI Pharmatech, III Annais of the SBTF Annual Meeting. Recife, Brazil: UFPE; 2001. p. 165-6.

  11. Mota EF. Atividade adjuvante dos óleos de amendoim (Arachis hypogaea L), algodão (Gossipium hirsutum L) e arroz (Oryza sativa L) na resposta imune humoral e celular, Ph.D. Thesis, Departamento de Bioquímica e Biología Molecular, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil; 2006.

  12. Moncada C, Torres V, Israel Y. Simple method for the preparation of antigen emulsions for immunization. J Immunol Methods1993;162:133-40.

  13. Becher P. Testing and emulsion properties, Emulsions Theory and Practice, second ed. New York: Reinhold; 1965

  14. Aroeira LS, Cardillo F, De Albuquerque DA, Vaz NM, Mengel J. Anti-IL-10 treatment does not block either the induction or the maintenance of orally induced tolerance to OVA. Scand J Immunol 1995; 41:319-23.

  15. Albuquerque DA, Saxena V, Adams DE, Boivin GP, Brunner HI, Witte DP. et al. An ACE inhibitor reduces Th2 cytokines and TGF-b1 and TGF-b2 isoforms in murine lupus nephritis. Kidney Int 2004;65:846-59.

  16. Snapper CM, Finkelman FD, Paul WE. Regulation of IgG1 and IgE production by interleukin 4. Immunol Rev 2004;102:51-75.

  17. McKenzie GJ, Fallon P, Emsom CL, Grencis R, McKenzie AN. Simultaneous disruption of interleukin (IL)-4 and IL-13 defines individual roles in T helper cell type 2-mediated responses. J Exp Med 1999;189:1565-72.

  18. Brewer JM, Conacher M, Satoskar A, Bluethmann H, Alexander J. Interleukin-4-deficient mice, alum not only generates T helper 1 responses equivalent to freund’s complete adjuvant, but continues to induce T helper 2 cytokine production. Eur J Immunol 1996;26:2062-6.

  19. Grun JL, Maurer PH. Different T helper cell subsets elicited in mice utilizing two different adjuvant vehicles: the role of endogenous interleukin 1 in proliferative responses. Cell Immunol 1989;121:134-45.

  20. Silva AS, Cavalcante LT, Faquim-Mauro EL, Macedo MS. Regulation of anaphylactic IgG1 antibody production by IL-4 and IL-10. Int Arch Allergy Immunol 2006;141:70-8.

  21. Broderson JR. A retrospective review of lesions associated with the use of Freund’s adjuvant. Lab Anim Sci 1989;39:400-5.

  22. Claasen E, Leeuw W, Greve P, Hendriksen C, Boersma W. Freund’s complete adjuvant: an effective but disagreable formula. Res Immunol 1992;143:478-83.

  23. Mbow ML, Gregorio E, Valiante NM, Rappuoli R. New adjuvants for human vaccine, Current Opinion in Immunol 2010;22:411- 6.

  24. Amyx HL. Control of animal pain and distress in antibody production and infectious disease studies. J Am Vet Med Assoc 1987; 191:1287-9.

  25. Lambrecht BN, Kool M, Willart MAM, Hammad H. Mechanism of action of clinically approved adjuvants. Current Opinion in Immunology 2009; 21:23-9.

  26. Reed SG, Bertholet S, Coler RN, Friede M. New horizons in adjuvants for vaccine development. Trends in Immunol 2009;30:23-32.

  27. Moro E, Junqueira JOB. Levantamento da incidência de reações vacinais e/ou medicamentosas em carcaças de bovinos ao abate em frigoríficos no Brasil. A hora Vet 1999;122:74-7.

  28. França AT, Alves GG, Mesquita AJ, Chiquetto CE, Bueno CP, Oliveira ASC. Perdas econômicas por abscessos vacinais e/ ou medicamentosos em carcaças de bovinos abatidos no estado de Goiás. Ciência Animal Brasileira 2006;7(1):93-6.

  29. Hilleman MR, Woodhour AF, Friedman A, Weibel RE, Stokes J. The clinical application of adjuvant-65. Ann Allergy 1972;30:152-8.

  30. Silva ACM, Guerra JL, Melo DF, Lima MS. Adjuvant effect of corn and olive oils in mouse immune response. In: VI Pharmatech, III Annais of the SBTF Annual Meeting. Recife, Brazil: UFPE; 2001. p. 165-6.

  31. Mota EF. Atividade adjuvante dos óleos de amendoim (Arachis hypogaea L), algodão (Gossipium hirsutum L) e arroz (Oryza sativa L) na resposta imune humoral e celular, Ph.D. Thesis, Departamento de Bioquímica e Biología Molecular, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil; 2006.

  32. Moncada C, Torres V, Israel Y. Simple method for the preparation of antigen emulsions for immunization. J Immunol Methods1993;162:133-40.

  33. Becher P. Testing and emulsion properties, Emulsions Theory and Practice, second ed. New York: Reinhold; 1965

  34. Aroeira LS, Cardillo F, De Albuquerque DA, Vaz NM, Mengel J. Anti-IL-10 treatment does not block either the induction or the maintenance of orally induced tolerance to OVA. Scand J Immunol 1995; 41:319-23.

  35. Albuquerque DA, Saxena V, Adams DE, Boivin GP, Brunner HI, Witte DP. et al. An ACE inhibitor reduces Th2 cytokines and TGF-b1 and TGF-b2 isoforms in murine lupus nephritis. Kidney Int 2004;65:846-59.

  36. Snapper CM, Finkelman FD, Paul WE. Regulation of IgG1 and IgE production by interleukin 4. Immunol Rev 2004;102:51-75.

  37. McKenzie GJ, Fallon P, Emsom CL, Grencis R, McKenzie AN. Simultaneous disruption of interleukin (IL)-4 and IL-13 defines individual roles in T helper cell type 2-mediated responses. J Exp Med 1999;189:1565-72.

  38. Brewer JM, Conacher M, Satoskar A, Bluethmann H, Alexander J. Interleukin-4-deficient mice, alum not only generates T helper 1 responses equivalent to freund’s complete adjuvant, but continues to induce T helper 2 cytokine production. Eur J Immunol 1996;26:2062-6.

  39. Grun JL, Maurer PH. Different T helper cell subsets elicited in mice utilizing two different adjuvant vehicles: the role of endogenous interleukin 1 in proliferative responses. Cell Immunol 1989;121:134-45.

  40. Silva AS, Cavalcante LT, Faquim-Mauro EL, Macedo MS. Regulation of anaphylactic IgG1 antibody production by IL-4 and IL-10. Int Arch Allergy Immunol 2006;141:70-8.

  41. Broderson JR. A retrospective review of lesions associated with the use of Freund’s adjuvant. Lab Anim Sci 1989;39:400-5.

  42. Claasen E, Leeuw W, Greve P, Hendriksen C, Boersma W. Freund’s complete adjuvant: an effective but disagreable formula. Res Immunol 1992;143:478-83.

  43. Mbow ML, Gregorio E, Valiante NM, Rappuoli R. New adjuvants for human vaccine, Current Opinion in Immunol 2010;22:411- 6.

  44. Amyx HL. Control of animal pain and distress in antibody production and infectious disease studies. J Am Vet Med Assoc 1987; 191:1287-9.

  45. Lambrecht BN, Kool M, Willart MAM, Hammad H. Mechanism of action of clinically approved adjuvants. Current Opinion in Immunology 2009; 21:23-9.

  46. Reed SG, Bertholet S, Coler RN, Friede M. New horizons in adjuvants for vaccine development. Trends in Immunol 2009;30:23-32.

  47. Moro E, Junqueira JOB. Levantamento da incidência de reações vacinais e/ou medicamentosas em carcaças de bovinos ao abate em frigoríficos no Brasil. A hora Vet 1999;122:74-7.

  48. França AT, Alves GG, Mesquita AJ, Chiquetto CE, Bueno CP, Oliveira ASC. Perdas econômicas por abscessos vacinais e/ ou medicamentosos em carcaças de bovinos abatidos no estado de Goiás. Ciência Animal Brasileira 2006;7(1):93-6.

  49. Hilleman MR, Woodhour AF, Friedman A, Weibel RE, Stokes J. The clinical application of adjuvant-65. Ann Allergy 1972;30:152-8.

  50. Silva ACM, Guerra JL, Melo DF, Lima MS. Adjuvant effect of corn and olive oils in mouse immune response. In: VI Pharmatech, III Annais of the SBTF Annual Meeting. Recife, Brazil: UFPE; 2001. p. 165-6.

  51. Mota EF. Atividade adjuvante dos óleos de amendoim (Arachis hypogaea L), algodão (Gossipium hirsutum L) e arroz (Oryza sativa L) na resposta imune humoral e celular, Ph.D. Thesis, Departamento de Bioquímica e Biología Molecular, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil; 2006.

  52. Moncada C, Torres V, Israel Y. Simple method for the preparation of antigen emulsions for immunization. J Immunol Methods1993;162:133-40.

  53. Becher P. Testing and emulsion properties, Emulsions Theory and Practice, second ed. New York: Reinhold; 1965

  54. Aroeira LS, Cardillo F, De Albuquerque DA, Vaz NM, Mengel J. Anti-IL-10 treatment does not block either the induction or the maintenance of orally induced tolerance to OVA. Scand J Immunol 1995; 41:319-23.

  55. Albuquerque DA, Saxena V, Adams DE, Boivin GP, Brunner HI, Witte DP. et al. An ACE inhibitor reduces Th2 cytokines and TGF-b1 and TGF-b2 isoforms in murine lupus nephritis. Kidney Int 2004;65:846-59.

  56. Snapper CM, Finkelman FD, Paul WE. Regulation of IgG1 and IgE production by interleukin 4. Immunol Rev 2004;102:51-75.

  57. McKenzie GJ, Fallon P, Emsom CL, Grencis R, McKenzie AN. Simultaneous disruption of interleukin (IL)-4 and IL-13 defines individual roles in T helper cell type 2-mediated responses. J Exp Med 1999;189:1565-72.

  58. Brewer JM, Conacher M, Satoskar A, Bluethmann H, Alexander J. Interleukin-4-deficient mice, alum not only generates T helper 1 responses equivalent to freund’s complete adjuvant, but continues to induce T helper 2 cytokine production. Eur J Immunol 1996;26:2062-6.

  59. Grun JL, Maurer PH. Different T helper cell subsets elicited in mice utilizing two different adjuvant vehicles: the role of endogenous interleukin 1 in proliferative responses. Cell Immunol 1989;121:134-45.

  60. Silva AS, Cavalcante LT, Faquim-Mauro EL, Macedo MS. Regulation of anaphylactic IgG1 antibody production by IL-4 and IL-10. Int Arch Allergy Immunol 2006;141:70-8.




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