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Revista Latinoamericana de Microbiología

2001, Number 4

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Microbiología 2001; 43 (4)

Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León

Salinas-Meléndez JA, Galván GS, Riojas-Valdés VM, Wong GA, Ávalos RR

Language: English
References: 22
Page: 161-164
PDF size: 75.85 Kb.


ABSTRACT

The aim of the present study was to determine the presence of Borrelia burgdorferi antibodies in horses from the metropolitan area of Monterrey, Nuevo León, México. Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. All sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed by Western blot. The serological test yielded 34 positive sera at 1:64 dilution, and from them 6 were positive at 1:128 dilution, 3 at 1:256, and only one at 1:512. Confirmation of the infection by Western blot was obtained only in the sample positive at the 1:512 dilution. These results shown a low frequency of seropositivity to B. burgdorferi of the horses in the area, confirming previous studies indicating that in northeast Mexico Lyme disease is present in different animal species.


REFERENCES

  1. Anderson, J.F. 1988. Mammalian and avian reservoirs for Borrelia burgdorferi. Ann. NY Acad. Sci. 539:180-191.

  2. Bosler, E.M., Ormistom, B.G., and Coleman, J.L. 1984. Prevalence of the Lyme disease spirochete in populations of white tailed Deer and white-footed mice. Yale J. Biol. Med. 57:651-659.

  3. Brock, D.T., and Madigan. M.T. 1993. Microbiología. 6a edición. Prentice Hall Hispanoamericana S.T. México, D.F.

  4. Burgess, E.C. 1988. Borrelia burgdorferi infection in Wisconsin horses and cows. Ann NY Acad Sci. 539:235-243.

  5. Burgess, E.C., Gendron-Fitzpatrick, A., and Wright, W.O. 1987. Arthritis and Systemic disease caused by Borrelia burgdorferi infection in a cow. J. Am. Vet. Med. Assoc. 191:1468-1470.

  6. Burgess, E.C., and Mattison, M. 1987. Encephalitis associated with Borrelia burgdorferi infection in a horse. J. Am. Vet. Med. Assoc. 191:1457-58.

  7. Burgess, E., Gillete, D., and Pickett, J.P. 1986. Arthritis and panuveitis as manifestations of Borrelia burgdorferi infection in a Wisconsin pony. J. Am. Vet. Med. Assoc. 189:1340-42.

  8. Cohen, N.D., Heck, F.C., Heim, B., Flad, D.M., Bosler, E.M., and Cohen, D. 1992. Seroprevalence of antibodies to Borrelia burgdorferi in a population of horses in Central Texas. J. Am. Vet. Med. Assoc. 201:1030-1034.

  9. Karlsson, M., Mollegard, I., Sternsted, G., and Wretlind, B. 1989. Comparison of Western blot and enzyme linked immunosorbent assay for diagnosis of Lyme borreliosis. Eur. J. Clin. Microbiol. Infect. Dis. 8:871-877.

  10. Lindenmayer, J., Weber, M., and Onderdonk, A. 1989. Borrelia burgdorferi infection in horses. J. Am. Vet. Med. Asoc. 194:1384.

  11. Magnarelli, L.A., and Anderson, F. 1989. Class-specific and polyvalent enzyme-linked immunosorbent assay for detection of antibodies to Borrelia burgdorferi in equids. J. Am. Vet. Med. Assoc. 195:1365-68.

  12. Magnarelli, L.A., Anderson, J.F., and Chappell, W.A. 1984. Antibodies to spirochetes in white tailed deer and prevalence of infected ticks from foci of Lyme disease in Connecticut. J. Wildl. Dis. 20:21-26.

  13. Magnarelli, L.A., Anderson, J.F., Kaufmann, A.F., Lieberman, L. L., and Withney, G.D. 1985. Borreliosis in dogs from Southern Connecticut. J. Am. Vet. Med. Assoc. 186:955-959.

  14. Magnarelli, L.A., Anderson, J.F., Shaw, E., Post, J.E., and Palka, F. 1988. Borreliosis in equids in Northeastern United States. J. Vet. Res. 49:359-362.

  15. Magnarelli, L.A., Flavell, R.A., Padula, S.J., Anderson, J.F., and Fikrig, E. 1997. Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays. J. Clin. Microbiol. 53:169-173.

  16. Marcus, L.C., Patterson, M.M., Eilpillan, R.E. and Urban, P. H. 1985. Antibodies to Borrelia burgdorferi in New England Horses: serological survey. Am. J. Vet. Res. 46:2570.

  17. Martínez, A., Salinas, A., Martínez, F., Cantú, A., and Miller, D.K. 1999. Serosurvey for selected diseases agents in white-tailed deer for Mexico. J. Wildlife Dis. 33 (4): 799-808.

  18. Parker, J.L., and White, K.K. 1992. Lyme Borreliosis in Cattle and Horses: a review of the literature. Cornell Vet. 82:253-274.

  19. Post, J.E., Shaw, E., and Palka, F. 1987. Lyme disease in horses. Proceeding of the American Association of Equine Practitioners. 32:415-485.

  20. Salinas-Meléndez, J.A., Tamez-González, R., Welsh-Lozano, O., and Barrera-Saldana, H.A. 1995. Detection of Borrelia burgdorferi DNA in humans skins biopsies and dog synovial fluid by the polymerase chain reaction. Rev. Lat.-Amer. Microbiol. 37:7-10.

  21. Salinas-Meléndez, J.A., Ávalos-Ramírez, R., Riojas-Valdez V.R., and Martínez-Muñoz, A. 1999. Serological survey of Canine Borreliosis. Rev. Lat.-Amer. Microbiol. 41:1-3.

  22. Steere A.C., Malawista, S.E., and Snydman, D.R. 1977. Lyme arthritis: an epidemic of oligoarticular arthritis in children and adults in three communities. Arthritis Rheum. 20:7-17.




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