medigraphic.com
ISSN 1561-3070 (Electronic)

2012, Number 1

<< Back Next >>

Rev Cub Oftal 2012; 25 (1)

Confocal microscopy results of one hundred healthy eye corneas

Gómez CZ, Fernández GK, Pérez TA, Márquez VS, Jareño OM, Cuevas RJ

Language: Spanish
References: 14
Page:
PDF size: 68.38 Kb.


ABSTRACT

Objective: This paper is aimed at analyzing the corneal cellular structures through Confoscan S4-aided confocal microscopy in apparently healthy corneas.
Methods: A prospective longitudinal study of 100 healthy eyes from practicing doctors, and from patients who had attended the corneal service at “Ramón Pando Ferrer” Cuban Institute of Ophthalmology in Havana since May 2007 was conducted. Both eyes of participating doctors were examined whereas the non-affected eye was examined in the patients. A total of 50 cases with no corneal disease notified from May 2007 to May 2008 were considered.
Results: The 100 studied eyes came from apparently healthy patients, and the pachymetry values of 64 of them were above the average. Since they were healthy corneas, the 3 types of epithelial cells as well as the keratocytes were present in almost all the patients and at different depths of the corneal stroma, respectively. Most of the studied eyes had endothelial cell counts above 2 500, a figure within the normal range, and there were nerve fibers in each of their layers.
Conclusion: Confocal microscope is a new tool that allows in vivo observation of corneal histology and thus supplements conventional biomicroscopy observations. It contributes to better understanding of corneal pathology, with a view to prophylactically and therapeutically acting upon corneal pathologies during their follow-up and evolution.


REFERENCES

  1. Legeais JM, Labetoulle M, Renard G, Gaillot D, Pouliquen Y. Indications for penetrating keratoplasty. A retrospective study of 2,962 cases over 11 years. J Fr Ophthalmol. 1993;16(10):516-22.

  2. Fonolla Gil M, Baamonde Arbaiza MB, Villacampa Castro T, González Castaño T, González Castaño C, Sánchez Aparicio JA. Resultados y complicaciones de las queratoplastias penetrantes. Arch. Soc. Esp. Oftalmol. 1999;74(10):513-58.

  3. Petroll MW, Mathew P, Cavanagh D, Qwight D, Jester JV. Clinical confocal microscopy. Curr Opin Ophthalmol. 1998;9(4):59-65.

  4. Kaufman SC, Musch DC, Belin MW, Cohen EJ, Meisler DM, Reinhart WJ, et al. Confocal microscopy: a report by the American Academy of Ophthalmology. Ophthalmology. 2004;111:396-406.

  5. Charles J, Koester CJ, Auran JD, Rosskothen HD, Flokaris GJ, Tackaberry RB. Clinical microscopy of the cornea utilizing optical sectioning and a high-numerical-aperture objective. J Opt Soc Am. 1993;10(7):1670-9.

  6. Ivarsen A, Stultiens BA, Moller-Pedersen T. Validation of confocal microscopy through focusing for corneal sublayer pachymetry. Cornea. 2002;21(7):700-4.

  7. Javaloy J, Vidal MT, Villada JR, Artola A, Alió JL. Comparison of four corneal pachymetry technique´s in corneal refractive surgery. J Refract Surg. 2004;20(1):29-34.

  8. Hahnel C, Somodi S, Weiss DG, Guthoff R. The keratocite network of human cornea: a three-dimensional study using confocal laser scanning fluorescence microscopy. Cornea. 2000;19(2):185-93.

  9. Linna T, Tervo T. Real-time confocal microscopic observations on human corneal nerves and wound healing after excimer laser photorefractive keratectomy. Curr Eye Res. 1997;16(7):640-9.

  10. Garin Ferreira R, Nebro Cobos S, Jimenez Pertiñez F, Escudero Gomez J. Queratoplastia penetrante, nuestra experiencia. Arch Soc Esp Oftalmol. 1996;71(2):107-14.

  11. Muraine M, Sanchez C, Watt L, Retout A, Brasseur G. Long term results of penetrating keratoplasty. A 10 year plus retrospective study. Graefes Arch Clin Exp Ophthalmol. 2003 Jul;241(7):571-6.

  12. Kucukerdonmez C, Akova YA, Dursun D. Refractive outcome of single running suture adjustment in penetrating keratoplasty. Eur J Ophthalmol. 2004 Mar Apr;14(2):94-9.

  13. Hernandez-Quintela E, Mayer FM, Dighiero P, Briat B, Savoldelli M, Legeais JM, et al. Confocal microscopy of cystic disorders of the corneal epithelium. Ophthalmology. 1998;105(4):631-6.

  14. Chiou AG, Kaufman SC, Beuerman RW, Ohta T, Soliman H, Kaufman HE. Confocal microscopy in cornea guttata and Fuchs´endothelial dystrophy. Br J Ophthalmol. 1999;83(2):185-9.




2020     |     www.medigraphic.com