2001, Number 3
Next >>
Microbiología 2001; 43 (3)
Antimicrobial sensitivity and adherence study in strains of coagulase-negative Staphylococcus spp.
Barberis IL, Pájaro MC, Godino SD, Pascual L, Daniele MD
Language: English
References: 21
Page: 109-113
PDF size: 61.77 Kb.
ABSTRACT
Antimicrobial sensitivity was evaluated from 120 strains of coagulase-negative
Staphylococcus (CoNS), isolated from urinary infections. The isolated species were identified by conventional methods and sensitivity to vancomycin, tetracyclin, norfloxacin, cephalothin erythromycin, clindamycin, oxacillin, penicillin, cyprofloxacin, ampicillin and ampicillin/sulbactam was tested by the agar dilution technique and the diffusion in disc technique. The distribution of the species was:
Staphylococcus epidermidis 26 (21.6%),
S. haemolyticus 48 (40.0%),
S. saprophyticus 40 (33.4%) and
S. simulans 6 (5%). CoNS strains extracted from urinary infections showed a high percentage of vancomycin and tetracyclin sensitivity, and a low sensitivity to Β-lactamic antibiotics, except ampicillin/sulbactam and cephalotin. Isolation percentage resistance to oxacillin was less than 48%. Strains resistant to oxacillin were considered as resistant to both penicillin and ampicillin. From 42% to 80% of
S. haemolyticus,
S. saprophyticus,
Staphylococcus epidermidis and
S. simulans were positive to the adherence test. Results from this work showed that slime producing CoNS strains, isolated in Río Cuarto Hospital, had different percentages of resistance to the studied antimicrobial agents.
REFERENCES
Akiyama, H., O. Yamasaki, H. Kanazaki, J. Tada, and J. Arata. 1998. Adherence characteristics of Staphylococcus aureus and coagulase-negative staphylococci isolated from various skin lesions. J. Dermatol. Sci. 18:132-136.
Baldassarri, L., G. Donelli, A. Gelosia, M. C. Voglino, S. W. Simpson, and G. D. Christensen. Purification and characterization of the Staphylococcal slime-associated antigen and its occurrence among Staphylococcus epidermidis clinical isolates. Infect. Immunol. 64:3410-3415.
Christensen, G. D., A. Simpson, A. Bisno, and E. Beachey. 1982. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect. Immun. 37:318-326.
Del’Alamo, L., R. F. Cereda, I. Tosin, E. A. Miranda, H. S. Sader. 1999. Antimicrobial susceptibility of coagulase-negative staphyloccoci and characterization of isolates with reduced susceptibility to glycopeptides. Diagn. Microbiol. Infect. Dis. 34:185-191.
Domaracki B. E., K. E. Preston, and R. A. Venezia. 1998. Increased oxacillin activity associated with glycopeptides in coagulase-negative staphylococci. Eur. J. Clin. Microbiol. Infect. Dis. 17:143-150.
Drozenova, J., P. Oetras. 2000. Characteristics of coagulase-negative staphylococci isolated from hemocultures. Epidemiol. Mikrobiol. Inmunol. 49:51-58.
Garrett, D. O., E. Jochimsen, B. Zimmer, and W. R. Jarvis. 1999. The emergence of decreased susceptibility to vancomycin in Staphylococcus epidermidis. Infect. Control. Hosp. Epidemiol. 20: 167-170.
Hubner, J., A. Kropec. 1995. Cross infections of coagulase-negative staphylococci in high-risk patients. Zentralbl. Bakteriol. 283:169-174.
Kleeman, K., T. L. Bannerman, W. E. Kloos. 1993. Species distribution of coagulase-negative staphylococcal isolates at a community hospital and implications for staphylococcal identification procedures. J.Clin. Microbiol. 31:13-18.
Kloos W. E., and D. W. Lambe Jr. Staphylococcus. 1991. In: Balows, Hamler, Hcrrmann, Isenberg, Shadomy (Ed.). Manual of Clinical Microbiology, 5th edition. American Society for Microbiology, Washington. Pp. 222-237.
Krcmery, V. Jr., J. Trupl, L. Drgona, J. Lacka, E. Kukuckova, and E. Oravcova. 1996. Nosocomial bacteremia due to vancomycin-resistant Staphylococcus epidermidis in four patients with cancer, neutropenia, and previous treatment with vancomycin. Eur. J. Clin. Microbiol. lnfect. Dis. 15:259-261.
Laverdiere, M. K. Weiss, R. Rivest, and J. Delorme. 1998. Trends in antibiotic resistance of staphylococci over an eight-year period: differences in the emergence of resistance between coagulase positive and coagulase- negative staphylococci. Microb. Drug. Resist. 4:119-122.
Nayak, N., and G. Satpathy. 2000. Slime production as a virulence factor in Staphylococcus epidermidis isolated from bacterial keratitis. Indian J. Med. Res. 111:6-10.
NCCLS. Performance standards for antimicrobial susceptibility testing. Documents: M2-T4 & M7-A2, 1998; M100-S3, 1991. NCCLS, Vilanova, Pa, USA.
Pinna, A, S. Zanetti, L. Sotgiu, L. A. Sechi, G. Fadda, and F. Carta. 1999. Identification and antibiotic susceptibility of coagulase negative staphylococci isolated in corneal/external infections. Br. J. Ophthalmol. 83:771-773.
Sieradzki, K., P. Villari, and A. Tomasz. 1998. Decreased susceptibilities to teicoplanin and vancomycin among coagulase-negative methicillin-resistant clinical isolates of staphylococci. Antimicrob. Agents Chemother. 42:100-107.
Strausbaught, L. J. 1999. Vancomycin-intermediate Staphylococcus epidermidis: curio or omen? Infect. Control. Epidemiol. 20:163-5
Sung L, et al. 1999. Bacteriemia due to persistent strains of coagulase-negative staphylococci in a neonatal intensive-care unit. Infect. Control Hosp. Epidemiol. 20:349-351.
Tenover, F.C., M. V. Lancaster, and B. C. Hill. 1998. Characterization of staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J. Clin. Microbiol. 36:1020-1027.
Veenstra, G.; Cremers, F.; Dijk, H. and Fleer, A. 1996. Ultrastructural organization and regulation of a biomaterial adhesin of Staphylococcus epidermidis. J. Bacteriol. 178:537-541.
Vu-Thien, H. 1998. Antibiotic sensitivity to isolated bacteria in pediatric urinary tract infections. Arch. Pediatr. 3:266S-268S.