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Revista Latinoamericana de Microbiología

2001, Number 2

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Microbiología 2001; 43 (2)

Evaluation of the Reveal

Robles-Reyes R, Eusebio-Hernández MG, Avilés-Ruiz D

Language: English
References: 36
Page: 76-83
PDF size: 109.28 Kb.


ABSTRACT

Salmonella belongs to the Enterobacteriaceae family, and is the cause of illnesses such as enteric fevers, enteritis and septicemia. Accordingly, a necessity exists of researching and developing new techniques, -which must be sensible, specific, economic and fast- for genus determination; all this related to the culture technique (official technique). In the present work, 80 samples of chicken were analyzed for Salmonella both by the Reveal™ equipment and by the culture technique with and without a pre-enrichment step (reference technique). Samples were collected in established markets in Netzahualcóyotl City and Chimalhuacán, Estado de México; and analyzed by the culture technique, from which 15 Salmonella strains were isolated. 14 of those strains were obtained when the pre-enrichment step was implemented. Meanwhile, using the Reveal™ equipment (new technique), the presence of 16 Salmonella strains was identified. The statistical method used, defined as a method of predictive values, calculates the sensitivity and specificity of the Reveal™ test in relation with the culture technique. It also determines the positive predictive value (PPV), that represents the probability that the microorganism is truly present when the Reveal™ test scores positive; and the negative predictive value (NPV), that represents the probability that the microorganim is not present when the Reveal™


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