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2012, Number 1

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VacciMonitor 2012; 21 (1)

Assessment of a preservation strategy for Brevundimonas diminuta

Burguet N, Sierra N

Language: Spanish
References: 19
Page: 10-13
PDF size: 137.19 Kb.


ABSTRACT

Brevundimonas diminuta is internationally recognized as the standard micro-organism for bacterial challenge tests. The Quality Control Laboratory of Liorad Laboratories has a collection of reference strains, among them Brevundimonas diminuta are kept by the freezing method at -70 0C, replicated in Lactose Broth Saline (CLS) culture medium. This methodology was not the most convenient, that is why another strategy was assessed. Triptone Soy Broth was used as growth method, skim milk 20% as protective substance and lyophilization as preservation method. An adequate quality control, which included purity verification, viability and stability of the properties, was carried out. Results obtained during the 24 months of the stability study in real time, suggest that the chosen method would provide an alternative and solution to the problem of this strain preservation.


REFERENCES

  1. Morales YE, Duque E, Rodríguez O, De la Torre J, Martínez RD, Pérez R, et. al. Bacterias Preservadas, una fuente importante de recursos biotecnológicos. Rev. Biotecnología Aplicada 2010;4(2):11-29.

  2. Floccari M. Métodos de conservación de cultivos bacterianos. Rev Argent Microbiol 1998;30:42-51.

  3. American Type Culture Collection. Certificare of Analysis Microbiology Lyophilized Microorganism Specifications and Performance Certificate Upon Initial Release. Brevundimonas diminuta ATCC 19146. Manassas, Virginia, EEUU: American Type Culture Collection; 2003

  4. ATCC Technical Bulletin No. 6 Reference strains: How many passages are too many? Reprinted from ATCC Connection 2003; 23(2):6-7.

  5. Jordan G. Micro-Filtration in the Production of Parenterals. Bull Parenteral Drug Assoc 1959;13(2):21-4.

  6. Fennington GJ, Howar G. Preparation and evaluation of bacterial stock for filter Validation. PDA. Journal of Pharmaceutical Science and Technology 1997;51(3):153-7.

  7. World Federation of Culture Collections. World Federation for Culture Collections Guidelines for the establishment and operation of collections of cultures of microorganisms. 3rd ed. Manassas,Virginia, EEUU: World Federation of Culture Collections; 2010.

  8. Palazon Z, Delgado AM. Estudio y desarrollo de un método de conservación de cepas. V Taller sobre Colecciones de Cultivos Microbianos. La Habana: Finlay Ediciones; 2006. Disponible en: http://www.finlay.sld.cu/ediciones.htm

  9. Snell JJ. General Introduction to Maintenance Methods. En: Kirsop BE, Doyle A. (eds.). Maintenance of Microorganisms and Cultured Cells. A Manual of Laboratory Methods. 2da edición. London: Academic Press; 1991. p. 21-30.

  10. Hawsworth DL, Sastramihardja I, Kokke R, Stevenson R. Guidelines for the Establishment and operation of Collections of Cultures of Microorganisms. WFCC Standards Committee. Richmond Surrey, UK: Simwort Press; 1990.

  11. Weng Z, Díaz OE, Álvarez I. Conservación de microorganismos: ¿qué debemos conocer? Rev Cubana Hig Epidemiol 2005;43(3):1-7.

  12. Moreira T, Gutiérrez A, Delgado H. Aspectos físicos relacionados con los aditivos en el proceso de liofilización. Papel relevante de los carbohidratos. Biotecnología Aplicada 1994;11(2):113-7.

  13. Stell KJ, Ross HE. Survival of freeze dried bacterial cultures. J Appl Microbiol 2008; 26(3):370-5.

  14. Collins CH. Métodos Microbiológicos. España: Editorial Acribia; 1969.

  15. Liofilchem Diagnostici. Integral System Enterobatteri Ref.71714; Code F00113: Colorimetric System for biochemical indentification and susceptibility testing of enterobacteria. Roseto DA, Italy: Liofilchem Diagnostici; 2004.

  16. Rojas N, Coto O, Pazos V. Manual de Prácticas de Laboratorio de Microbiología Clínica. Ciudad Habana: Editorial Pueblo y Educación; 1987.

  17. The United States Pharmacopeia, 30th ed. The National Formulary, 25th ed. USP30-NF25. Rockville: Mack Printing; 2007.

  18. Prieto M. Bacilos gramnegativos no fermentadores (monografía en Internet). Buenos Aires: Asociación Argentina de Microbiología; 2006. Disponible en: //www.aam.org.ar/ actividades/T2-3. pdf

  19. Escobar B, Llanes L, Parra M. Obtención de un stock de Brevundimonas diminuta utilizando un método alternativo de conservación. En: IV Taller sobre Colecciones de Cultivos Microbianos. La Habana: Ediciones Finlay; 2006. Disponible en: http://www.finlay.sld.cu/ediciones.htm




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