Marrero DD, Rodríguez LEA, González CVL, Adames FY, Vicente MR

Language: Spanish
References: 15
Page: 21-29
PDF size: 210.49 Kb.

ABSTRACT

Introduction: D004, a new lipid extract purified from the Cuban royal palm (Roystonea regia [Kunth] F. Cook) fruits, has been shown to be effective in experimental models of prostate hyperplasia, and to exhibit antioxidant and antiinflammatory effects.
Objectives: to identify the fatty alcohols that could be present in D004 active ingredient using gas chromatography-mass spectrometry.
Methods: samples of nine batches of D004 were submitted to saponification with KOH/EtOH solution and the unsaponifiable fractions were extracted with n-hexane. The alcohols were analyzed as trimetilsilil derivatives, which were obtained by reaction with MSTFA. The quantification was carried out by the internal standard method using 1-eicosanol.
Results: the total content of alcohols in D004 ranged from 0.18 to 5.10 %. This fraction was mainly composed of 1-octacosanol (52.94 %), 1-triacontanol (25.91 %), and 1-hexacosanol (14.40 %); whereas the minority components were 1-docosanol, 1-tricosanol, 1-tetracosanol, 1-pentacosanol, 1-heptacosanol, 1-nonacosanol, 1-dotriacontanol 1-tetratriacontanol, 1-tetradecanol, 1-pentadecanol, 1-hexadecanol, 1-octadecanol and 1-eicosanol were minor components, being the last six alcohols present in trace levels.
Conclusions: fatty alcohols were identified and quantified in D004 active ingredient by gas chromatography-mass spectrometry. The major components were 1-octacosanol, 1-triacontanol and 1-hexacosanol.

REFERENCES

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