Méndez RLA, Nodarse RA, Morales RE, Barrios MA, Soriano TM, Castelvi LA

Language: Spanish
References: 14
Page: 1-10
PDF size: 201.84 Kb.

ABSTRACT

Fluorescence in situ hybridization applied to the cytogenetic prenatal diagnosis is a rapid way to stablish a nexus between genes and chromosomes without celular culture and allows detection of chromosomal abnormalities on interphase cells. The aim of the present study was to evaluate this method as a tool in prenatal diagnosis of aneuploidies in high risk pregnancies. Prenatal diagnosis was carried out in 40 high-risk pregnancies using fluorescence in situ hybridization, 34 had successuful results. The 97 % the cases were confirmed by conventional cytogenetic results. The diagnosis of 18, 21 and 13 chromosome aneuploidies showed three hybridization signals in 80 % of the scored nuclei. The results of fluorescence in situ hybridization were in conformity with the results of cytogenetic analysis in all the normal cases (sex and autosomic chromosomes). This technique should be applied in high risk cases of chromosomes aneuploidies (21,18, 13 and X), high maternal anxiety, or when significant clinical situation is present. It should be employed as an adjunctive tool to the examination of fetal chromosomes.

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