Valdez SJJ, Ruiz AD, Vázquez RS, Calzada GN, Guzmán TMG

Language: Spanish
References: 24
Page: 27-34
PDF size: 96.56 Kb.

ABSTRACT

Introduction: SD Dengue Duo (Standard Diagnosis) commercial kit is an immunochromatographic rapid test that detects NS1 protein and IgG/IgM dengue antibodies simultaneously.
Objective: to evaluate the operational and functional characteristics of this system for the detection of virological and serological markers. Methods: sera panel was made up by 161 samples, 113 from patients with clinically and serologically confirmed dengue caused by any of the four dengue virus serotypes and 48 negative samples. All these samples were tested by SD Dengue Duo Kit and by Platelia Dengue NS1 Ag, IgM Capture ELISA and ELISA Inhibition Method used as reference assays. Results: the evaluated kit showed a 57.75% sensitivity for the detection of NS1 protein, false negatives were detected in samples collected 5 days or more after fever onset in secondary infection cases. IgM detection showed 96.0% sensitivity and 98.4% specificity. Furthermore, high agreement (95.7%) in classifying dengue infection types (primary or secondary infections) was observed. The global study of the 3 markers, the sensitivity rose to 100%. Conclusions: SD Dengue Duo is a simple, easy and rapid assay; it does not require additional equipment, can be used for acute and convalescence serum samples and offers a good alternative for dengue diagnosis in those laboratories where a complete dengue virus diagnosis is difficult to perform.

REFERENCES

1. Guzman MG, Kouri G. Dengue: an update. Lancet Infect Dis. 2002;2(1):33-42.

2. WHO. Dengue and dengue hemorrhagic fever. 2008[cited 5 Jun 2011]. Disponible en: http://www.who.int/mediacentre/ factsheets/fs117/en/

3. Guzmán Tirado MG, Rosario D, Kourí G. Molecular Biology of the Flavivirus. Norfolk: Horizon Bioscience; 2006. p. 191-223.

4. Buchy F, Yoksan S, Peeling RW, Hunsperger E. Laboratory tests for the diagnosis of dengue virus infection. Geneva, Switzerland: TRD/ Scientific Working Group; 2006. p. 74-85.

5. Senanayake S. Dengue fever and dengue haemorrhagic fever—a diagnostic challenge. Aust Fam Physician. 2006;35(8):609-12.

6. Guzman M, Garcia G, Kouri G. Dengue and dengue hemorrhagic fever: research priorities. Rev Panam Salud Publica. 2006;19(3):204-15.

7. Young PR, Hilditch PA, Bletchly C, Halloran W. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol. 2000;38(3):1053-7.

8. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M. Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol. 2002;40(2):376-81.

9. Vázquez S, Ruiz D, Barrero R, Ramírez R, Calzada N, Del Rosario Peña B, et al. Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients. Diagnostic Microbiology Infectious Disease. 2010;68(46-9).

10. Bessoff K, Phoutrides E, Delorey M, Acosta L, Hunsperger E. Utility of a commercial nonstructural protein 1 antigen capture kit as a dengue virus diagnostic tool. Clin Vaccine Immunol. 2010;17(6):949-53.

11. Guzmán MG, Jaenisch T, Gaczkowski R, Ty Hang VT, Sekaran SD, Kroeger A, et al. Multi-country evaluation of the sensitivity and specificity of two commercially-available NS1 ELISA assays for dengue diagnosis. PLoS Negl Trop Dis. 2010;4(8):e811.

12. Tricou V, Vu HT, Quynh NV, Nguyen CV, Tran HT, Farrar J, et al. Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses. BMC Infect Dis 2010; 10:142.

13. Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, DungNM, et al. Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. PLoS Negl Trop Dis. 2009;3(1):e360.

14. Vazquez S, Cabezas S, Perez AB, Pupo M, Ruiz D, Calzada N, et al. Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections. Int J Infect Dis. 2007;11(3):256-62.

15. Vazquez S, Acosta N, Ruiz D, Calzada N, Alvarez AM, Guzman MG. Immunoglobulin G antibody response in children and adults with acute dengue 3 infection. J Virol Methods. 2009;159(1):6-9.

16. Hunsperger EA, Yoksan S, Buchy P, Nguyen VC, Sekaran SD, Enria DA, et al. Evaluation of commercially available anti-dengue virus immunoglobulin M tests. Emerg Infect Dis. 2009;15(3):436-40.

17. Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, et al. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis. 2008;2(8):e280. 34

18. Dussart P, Labeau B, Lagathu G, Louis P, Nunes MR, Rodrigues SG, et al. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol. 2006;13(11):1185-9.

19. Libraty DH, Young PR, Pickering D, Endy TP, Kalayanarooj S, Green S, et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis 2002; 186(8):1165-8.

20. Blacksell SD, Doust JA, Newton PN, Peacock SJ, Day NP, Dondorp AM. A systematic review and meta-analysis of the diagnostic accuracy of rapid immunochromatographic assays for the detection of dengue virus IgM antibodies during acute infection. Trans R Soc Trop Med Hyg. 2006; 100(8):775-84.

21. Nga TT, Thai KT, Phuong HL, Giao PT, Hung Le Q, Binh TQ, et al. Evaluation of two rapid immunochromatographic assays for diagnosis of dengue among Vietnamese febrile patients. Clin Vaccine Immunol. 2007;14(6):799-801.

22. Halstead SB. Antibodies determine virulence in dengue. Ann N Y Acad Sci. 2009;1171(Suppl 1):E48-56.

23. Kouri GP, Guzman MG, Bravo JR. Why dengue haemorrhagic fever in Cuba? 2. An integral analysis. Trans R Soc Trop Med Hyg. 1987;81(5):821-3.

24. Guzman MG, Kouri G. Dengue haemorrhagic fever integral hypothesis: confirming observations, 1987-2007. Trans R Soc Trop Med Hyg. 2008;102(6):522-3.