Díaz GA; Rodríguez SH; Scull LR

Language: Spanish
References: 23
Page: 101-108
PDF size: 60.29 Kb.

ABSTRACT

OBJECTIVES: to evaluate the effect of 10 Cuban medicinal plant extracts on the human lung tumor cell line A549.
METHODS: the effect of the plant extracts on tumor cells was determined by a colorimetric assay using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) at concentrations ranging from 3,9-250 μg/mL for 72 hours and the mean cytotoxic concentration was calculated for each of them.
RESULTS: the ethanolic extracts of Parthenium hysterophorus, Bixa orellana, Momordica charantia and Cucurbita maxima showed mean cytotoxic concentrations under 100 μg/mL. Except for P. hysterophorus, the others are used in traditional medicine to fight cancer. The remaining extracts from Cecropia peltata, Melia azedarach, Annona glabra, Artemisia absintium, Lepidium virginicum and Bidens pilosa did not show significant cytotoxic effects.
CONCLUSIONS: the plant extracts for cancer treatment in traditional medicine showed cytotoxic effect on the tumor cell lines. Ethnobotanical data represent an important tool for medicinal plants screening in the quest for new compounds to treat cancer.

REFERENCES

1. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007;70(3):461-77.

2. Jinwoong K, Eun JP. Cytotoxic Anticancer Candidates from Natural Resources Current Medicinal Chemistry. Anti-Cancer Agents. 2002;2(4):485-537.

3. Roig JT. Plantas medicinales, aromáticas o venenosas de Cuba. La Habana: Editorial INRA; 1988.

4. Fuentes V, Expósito A. Las encuestas etnobotánicas sobre plantas medicinales en Cuba. Rev Jardín Botánico Nacional. 1995;16:77-144.

5. Beyna A, León MC, Iglesias E, Ferrádiz D, Herrera R, Volpato G, et al. Estudios etnobotánicos sobre plantas medicinales en la provincia de Camagüey (Cuba). Anal Jardín Botánico Madrid. 2004;61(2):185-204.

6. Ministerio de Salud Pública (MINSAP). Medicamentos de origen vegetal: extractos y tinturas: proceso tecnológico. Norma Ramal de Salud Pública (NRSP); 311. La Habana: MINSAP; 1991.

7. Mosmann T. Rapid colorimetric assay for cellular grow and survival: application to proliferation and citotoxicity assays. J Immunol Meth. 1983; 65:55-63.

8. Cordell GA, Kinghorn AD, Pezzuto JM. Separation, structure elucidation and bioassay of cytotoxic natural products, In: Colegate SM, Molyneux R. J. eds. Bioactive Natural Products: Detection, Isolation and Structure Determination. Boca Raton: CRC Press; 1993, 99-200.

9. Das B, Das R. Chemical investigation in Parthenium hysterophorus L. an allelopathic plant. Allelopathy J. 1995;2:99-104.

10. Belz RG. Stimulation Versus Inhibition-Bioactivity of Parthenin, A Phytochemical From Parthenium hysterophorus L. Dose Response. 2007; 6(1):80-96.

11. Reddy MK, Alexander-Lindo RL, Nair MG. Relative inhibition of lipid peroxidation, cyclooxygenase enzymes, and human tumor cell proliferation by natural food colors. J Agric Food Chem. 2005;53(23):9268-73.

12. Zegarra L, Vaisberg A, Loza C, Aguirre RL, Campos M, Fernandez I, et al. Double-blind randomized placebo-controlled study of Bixa orellana in patients with lower urinary tract symptoms associated to benign prostatic hyperplasia. Int Braz J Urol. 2007;33(4):493-500.

13. Akihisa T, Higo N, Tokuda H, Ukiya M, Akazawa H, Tochigi Y, et al. Cucurbitanetype triterpenoids from the fruits of Momordica charantia and their cancer chemopreventive effects. J Nat Prod. 2007;70:1233-9.

14. Ray R, Raychoudhuri A, Steele R, Nerurkar P. Bitter melon (Momordica charantia) extract inhibits breast cancer cell proliferation by modulating cell cycle regulatory genes and promotes apoptosis. Cancer Res. 2010;70:1925-31.

15. Grossmann ME, Mizuno NK, Dammen ML, Schuster T, Ray A, Cleary MP. Eleostearic acid inhibits breast cancer proliferation by means of an oxidationdependent mechanism. Cancer Prev Res. 2009;2:879-86.

16. Nerurkar P, Ray RB. Bitter Melon: Antagonist to Cancer. Pharm Res. 2010; 27:1049-53.

17. López AM, Rojas N, Jiménez CA. Actividad antineoplásica potencial de extractos de plantas que crecen en Cuba. Parte IV. Rev Cubana Farm. 1981; 15(1):71-7.

18. Jabit ML, Wahyuni FS, Khalid R, Israf DA, Haari K, Lajis NH, Stanslas J. Cytotoxic and nitric oxide inhibitory activities of methanol extracts of Garcinia species. Pharmaceutical Biol. 2009;47(11):1019-26

19. Kviecinski MR, Felipe KB, Schoenfelder T, Wiese LP de Lemos, Rossi MH, Gonçalez E, Felicio JD'arc et al. Study of the antitumor potential of Bidens pilosa (Asteraceae) used in Brazilian folk medicine. J Ethnopharmacol. 2008; 117(1):69- 75

20. Valdés HAL, Rego HPL. Bidens pilosa Linné. Rev Cubana Plant Med [revista en la Internet]. 2001 Abr [citado 2010 Nov 28]; 6(1): 28-33. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962001000100007&lng=es&nrm=iso&tlng=es

21. Cochrane CB, Nair PK, Melnick SJ, Resek AP, Ramachandran C. Anticancer Effects of. Annona glabra Plant Extracts in Human. Leukemia Cell Lines. Anticancer Res. 2008;28:965-71.

22. Nibret E, Wink M. Volatile components of four Ethiopian Artemisia species extracts and their in vitro antitrypanosomal and cytotoxic activities. Phytomedicine. 2010;17:369-74.

23. He L, Yin N, Cheng J, Wu X, Jiang J, Song X. Structural features of a new heteropolysaccharide from the fruit bodies of Melia azedarach and its effect on cytotoxic activity. Fitoterapia. 2009;80:399-403.