Oscar Javier Patiño Ladino, Juliet Angélica Prieto Rodríguez, José Manuel Lozano Moreno, Liliana Lesmes Sarmiento, Luís Enrique Cuca SLE

Language: Spanish
References: 29
Page: 431-438
PDF size: 55.79 Kb.

ABSTRACT

Currently, infectious diseases caused by different microorganisms are one of major causes of death worldwide, so the search for new antimicrobial agents has become indispensable, being the natural products one potential source to find antimicrobial compounds. The species of Zanthoxylum genus are characterized by different types of secondary metabolites, many of which exhibit interesting biological properties against several human pathogens. In this research work, the antibacterial activity of 21 substances isolated from two species of Zanthoxylum (Rutaceae) genus was evaluated. It was determined by agar radial diffusion method against standard strains of Gram (+) bacteria: Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212 and Gram (-) bacteria: Escherichia coli ATCC 25922 and Salmonella tiphymurium, strains ATCC 14028s and MS7953. Of the 21 natural substances evaluated, 11 caused some degree of bacterial growth inhibition. The benzophenanthridine alkaloid called chelerythrine showed the greatest antibacterial activity when inhibiting the growth of five strains with inhibition zones comparable or superior to those exhibited by the antibiotics used as positive controls. Isoquinoline alkaloids are the most promising substances for further specific studies of antibacterial activity; in addition to their interesting action, some of these substances are able to inhibit the growth of Gram (+) and Gram (-) bacteria, thus indicating their broad spectrum antibacterial action.

REFERENCES

1. Cragg GM, Kingston DGI, Newman DJ, editors. Anticancer agents from natural products. New York: Taylor & Francis Group; 2005:1-3.

2. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007;70:461-77.

3. Colegate SM, Molyneux RJ. An introduction and overview. En: Colegate SM, Molyneux RJ, editors. Bioactive natural products: detection, isolation, and structural determination. 2nd. ed. New York: CRC Press; 2008. p. 1-3.

4. Kaufman PB, Kirakosyan A, McKenzie M, Dayanandan P, Hoyt JE, Li C. The uses of plant natural products by humans and risks associated with their use. En: Cseke L, Kirakosyan A, Kaufman P, Warber S, Duke J, Brielmann H, editors. Natural products from plants. New York: CRC Taylor & Francis; 2006. p. 442-68.

5. Pan L, Chay H, Kinghorn AD. The Continuing search for antitumor agents from higher plants. Phytochem Lett. 2010;3:1-8.

6. Nwaka S, Hudson A. Innovative lead discovery strategies for tropical diseases. Nature Rev Drug Discov. 2006;5:941-55.

7. Segal E, Elad D. Fungal vaccines and immunotherapy. J Mycol Med. 2006;16:134-51.

8. Waldvogel F. Infectious diseases in the 21st century: old challenges and new opportunities. Int J Infect Dis. 2004;8:5-12.

9. Valgas C, Machado de Souza S, Smania E, Smania A. Screening methods to determine antibacterial activity of natural products. Braz J Microbiol. 2007;38:369-80.

10. Patrick G. An Introduction to medicinal chemistry. 2nd. ed. New York: Oxford University Press; 2001. p. 429-31.

11. Biswas S, Roult D, Rolain JA. Bioinformatic approach to understanding antibiotic resistance in intracellular bacteria through whole genome analysis. Int J Antimicrob. Ag. 2008;32:207-20.

12. Lobova T, Barkhatov Y, Salamatina O, Popova L. Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of lake shira as an indicator of human impact in the ecosystem. Microbiol Res. 2008;163:152-60.

13. Nuñez B, Salazar R. Uso racional de antibióticos. Módulo 2. Línea antibiótica- Bristol Mayers Squibb. Quito: Universidad Central de Ecuador; 2005. p. 4-5.

14. Benin AL, Dowell SF. Antibiotic resistance and implications for the appropiate use of antimicrobial agents. En: Mainous A, Pomeroy C, editors. Management of antimicrobials in infectious diseases: impact of antibiotic resistance. New York: Humana Press; 2001. p. 13-8.

15. McGaw LJ, Lall N, Meyer JJM, Eloff JN. The potential of south african plants against Mycobacterium infections. J Ethnopharmacol. 2008;119:482-500.

16. Rochfort S, Parker AJ, Dunshea FR. Plant bioactives for ruminant health and productivity. Phytochemistry. 2008;69:299-322.

17. Adesina SK. The Nigerian Zanthoxylum: chemical and biological values. Afr J Trad CAM. 2005;2:282-301.

18. Dieguez-Hurtado R, Garrido-Garrido G, Prieto-González S, Iznaga Y, González L, Molina-Torres J, Curini M, et al. Antifungal activity of some cuban Zanthoxylum species. Fitoterapia. 2003;74:384-86.

19. Patiño OJ, Cuca LE. Alcaloides Benzofenantridínicos de Zanthoxylum quinduensis. Rev Colomb Quim. 2004;33:13-20.

20. Waterman PG, Grundon MF. Chemistry and chemical taxonomy of the Rutales. London: Academic Press; 1983.

21. Patiño OJ, Cuca LE. Isoquinoline alkaloids of Zanthoxylum quinduense (Rutaceae). Biochem Sys Ecol. 2010;38:853-6.

22. Patiño OJ, Cuca LE. Chemical constituents of the wood of Zanthoxylum quinduense Tul. (Rutaceae). Quim Nova. 2010;33:1019-21.

23. Cuca LE, Martinez JC, Monache FD. Constituyentes químicos de Zanthoxylum monophyllum. Rev Colomb Quim. 1998;27:17-27.

24. Lehrer RI, Rosenman M, Harwig SSSL, Jackson R, Eisenhauer P. Ultrasensitive assay for endogenous antimicrobial polypeptides. J Inmunol Methods. 1991;137:167-73.

25. Weber AD. Estudo fitoquímico e da actividade biológica de Zanthoxylum rhoifolium. Tesis de Maestría. Centro de Ciencias Naturais e Exatas. Santa Maria, RS, Brasil: Universidade Federal de Santa Maria; 2005. p. 116-21.

26. Okunade AL, Elvin-Lewis MPF, Lewis WH. Natural antimycobacterial metabolites: current status. Phytochemistry. 2004;65:1017-32.

27. Newton SM, Lau C, Gurcha SS, Bersa GS, Wright CW. The evaluation of fortythree plant species for in vitro antimycobacterial activities; isolation of active constituents from Psoralea corylifolia and Sanguinaria canadiensis. J Ethnopharm. 2002;79:57-67.

28. Wang X, Yao X, Zhu Z, Tang T, Dai K, Sadovskaya I, Flahaut S, Jabbouri S. Effect of berberine on Staphylococcus epidermis biofilm formation. Int J Antimicrob Agents. 2009;34:60-6.

29. Iwasa K, Kamigauchi M, Ueki M, Taniguchi M. Antibacterial activity and structure-activity relationships of berberine analogs. Eur J Med Chem. 1996;3:469-78.