medigraphic.com
ISSN 1561-2988 (Print)

2011, Number 3

<< Back Next >>

Rev Cubana Farm 2011; 45 (3)

Association between clinical indicators of spermogram and redox variables in the male infertility

Mallok A, Martínez-Sánchez G, Flores-Sánchez RM, Alonso-Rodríguez CA

Language: Spanish
References: 38
Page: 361-379
PDF size: 241.52 Kb.


ABSTRACT

In addition to the conventional factors originating infertility, the oxidative stress has been identified as the causative factor of spermatozoid damage; however, the methods for its clinical determination have not been developed yet. The present study was aimed at evaluating the correlation among the global redox state indicators of samples of blood serum, erythrocyte lysin, seminal plasma and sperm lysin in order to identify the possible biomarkers. Samples taken from 31 patients diagnosed with male infertility and 30 apparently health volunteers were analyzed. It was confirmed that all the samples had indicators of biomolecule damage which increased significantly (p< 0,01) in patients compared with the control group. The damage indicators comprised low total antioxidant capacity levels in the lipid phase and imbalance in the activity of enzymes catalase and superoxide dismutase (catalase/superoxide dismustase ratio is 10 times higher in seminal plasma of patients when compared to that of the controls). The increase of total malonindialdehyde and organoperoxide measured in the seminal plasma correlated with the increase of sperm hypofunction indicators such as mobility and morphological characteristics. The behaviour of redox variables in the seminal plasma had great diagnostic value and was submitted as a proposal for use in the follow-up of nutritional or pharmacological interventions in these patients.


REFERENCES

  1. Saalu LC. The incriminating role of reactive oxygen species in idiopathic male infertility: an evidence based evaluation. Pak J Biol Sci [Internet] 2010 [cited2010 Nov 6]; 13(9):413-422. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20973394

  2. O'Flaherty C, de Lamirande E, Gagnon C. Reactive oxygen species and protein kinases modulate the level of phospho-MEK-like proteins during human sperm capacitation. Biol Reprod [Internet] 2005 [ cited 2010 Nov 1]; 73(1):94-105. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15772258

  3. Hung AJ, King P, Schlegel PN. Uniform testicular maturation arrest: a unique subset of men with nonobstructive azoospermia. J Urol [Internet] 2007 [cited 2010 Nov 16]; 178(2):608-612; discussion 612. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17570432

  4. de Lamirande E, Lamothe G. Levels of semenogelin in human spermatozoa decrease during capacitation: involvement of reactive oxygen species and zinc. Hum Reprod [Internet] 2010 [cited 2010 Nov 3]; 25(7):1619-1630. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20501468

  5. de Lamirande E, Lamothe G, Villemure M. Control of superoxide and nitric oxide formation during human sperm capacitation. Free Radic Biol Med [Internet] 2009 [cited 2010 Nov 1]; 46(10):1420-1427. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19268528

  6. Tremellen K. Oxidative stress and male infertility—a clinical perspective. Hum Reprod Update [Internet] 2008 [cited 2010 Nov 5]; 14(3):243-258. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18281241

  7. WMA. World Medical Association Declaration of Helsinki. Ethical Principles for Medical Research Involving Human Subjects. Adopted by the 18th WMA General Assembly, Helsinki, Finland, June 1964: J Int Bioethique. 2004;12:124-9.

  8. Kasimanickam R, Pelzer KD, Kasimanickam V, Swecker WS, Thatcher CD. Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs. Theriogenology [Internet] 2006 [cited 2010 Nov 1]; 65(7):1407-1421. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16188307

  9. Marklund S, ed. Pyrogallol autooxidation. Boca Raton, FL: CRC; 1985.

  10. Aebi H. Catalase in vitro. Methods Enzymol [Internet] 1984 [cited 2010 Nov 5]; 105:121-126. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6727660

  11. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol [Internet] 1990 [cited 2010 Nov 7]; 186:407-421. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2233308

  12. Nourooz-Zadeh J. Ferrous ion oxidation in presence of xylenol orange for detection of lipid hydroperoxides in plasma. Methods Enzymol [Internet] 1999 [cited 2010 Nov 7]; 300:58-62. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9919509

  13. Witko-Sarsat V, Nguyen-Khoa T, Jungers P, Drueke TB, Descamps-Latscha B. Advanced oxidation protein products as a novel molecular basis of oxidative stress in uraemia. Nephrol Dial Transplant [Internet] 1999 [cited 2010 Nov 9]; 14 Suppl1:76-78. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10048460

  14. Ozdemirler G, Mehmetcik G, Oztezcan S, Toker G, Sivas A, Uysal M. Peroxidation potential and antioxidant activity of serum in patients with diabetes mellitus and myocard infarction. Horm Metab Res [Internet] 1995 [cited 2010 Nov 5]; 27(4):194-196. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7750904

  15. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem [Internet] 1996 [cited 2010 Nov 6]; 239(1):70-76. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8660627

  16. Camera E, Picardo M. Analytical methods to investigate glutathione and related compounds in biological and pathological processes. J Chromatogr B Analyt Technol Biomed Life Sci [Internet] 2002 [cited 2010 Nov 9]; 781(1-2):181-206. Available from:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12450659

  17. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem [Internet] 1976 [cited 2010 Nov 3]; 72:248-254. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=942051

  18. Drabkin DL. The standardization of hemoglobin measurement. Am J Med Sci [Internet] 1949 [cited 2010 Nov 6]; 217(6):710. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18210406

  19. WHO. Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. 5 ed. Cambridge, UK: Cambridge University Press; [Internet] 2010 [cited 2010 Nov 6]. Available from: http://whqlibdoc.who.int/publications/2010/9789241547789_eng.pdf

  20. Marmor D, Grob-Menendez F. [Flagellar anomalies in routine spermatozoa cytograms]. J Gynecol Obstet Biol Reprod (Paris) [Internet] 1990 [cited 2010 Nov 6]; 19(2):181-185. Available from: http://www.ncbi.nlm.nih.gov/pubmed/2324440?dopt=Citation

  21. Politch JA, Wolff H, Hill JA, Anderson DJ. Comparison of methods to enumerate white blood cells in semen. Fertil Steril [Internet] 1993 [cited 2010 Nov 4]; 60(2):372-375. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8393411

  22. Aziz N, Agarwal A. Evaluation of sperm damage: beyond the World Health Organization criteria. Fertil Steril [Internet] 2008 [cited 2010 Nov 6]; 90(3):484-485. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17953944

  23. Aziz N, Said T, Paasch U, Agarwal A. The relationship between human sperm apoptosis, morphology and the sperm deformity index. Hum Reprod [Internet] 2007 [cited 2010 Nov 6]; 22(5):1413-1419. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17303629

  24. Thomas SR, Witting PK, Drummond GR. Redox control of endothelial function and dysfunction: molecular mechanisms and therapeutic opportunities. Antioxid Redox Signal [Internet] 2008 [cited 2010 Nov 6]; 10(10):1713-1765. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18707220

  25. Zalata AA, Christophe AB, Depuydt CE, Schoonjans F, Comhaire FH. The fatty acid composition of phospholipids of spermatozoa from infertile patients. Mol Hum Reprod [Internet] 1998 [cited 2010 Nov 6]; 4(2):111-118. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9542967

  26. Gliozzi TM, Zaniboni L, Maldjian A, Luzi F, Maertens L, Cerolini S. Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets. Theriogenology [Internet] 2009 [cited 2010 Nov 5]; 71(6):910-919. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19121864

  27. Hwang K, Lipshultz LI, Lamb DJ. Use of Diagnostic Testing to Detect Infertility. Curr Urol Rep [Internet] 2010 [cited 2010 Nov 6]. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21088937

  28. Blazovics A. [From free radicals to science of nutrition]. Orv Hetil [Internet] 2009 [cited 2010 Nov 8]; 150(2):53-63. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19103556

  29. Tavilani H, Doosti M, Saeidi H. Malondialdehyde levels in sperm and seminal plasma of asthenozoospermic and its relationship with semen parameters. Clin Chim Acta [Internet] 2005 [cited 2010 Nov 6]; 356(1-2):199-203. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15936318

  30. Rodrigues JK, Dib LA, Ferriani RA, Jordao Junior AA, Navarro PA. [Serum markers of oxidative stress and assisted reproduction procedures results in infertile patients with polycystic ovary syndrome and controls]. Rev Bras Ginecol Obstet [Internet] 2010 [cited 2010 Nov 7]; 32(3):118-125. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20512258

  31. Mahfouz RZ, du Plessis SS, Aziz N, Sharma R, Sabanegh E, Agarwal A. Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress. Fertil Steril [Internet] 2010 [cited 2010 Nov 1]; 93(3):814-821. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19100530

  32. Witko-Sarsat V, Gausson V, Descamps-Latscha B. Are advanced oxidation protein products potential uremic toxins? Kidney Int Suppl [Internet] 2003 [cited 2010 Nov 8]; (84):S11-14. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12694298

  33. Droge W, Schipper HM. Oxidative stress and aberrant signaling in aging and cognitive decline. Aging Cell [Internet] 2007 [cited 2010 Nov 6]; 6(3):361-370. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17517043

  34. Vernet P, Aitken RJ, Drevet JR. Antioxidant strategies in the epididymis. Mol Cell Endocrinol [Internet] 2004 [cited 2010 Nov 3]; 216(1-2):31-39. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15109742

  35. Bansal AK, Bilaspuri GS. Impacts of oxidative stress and antioxidants on semen functions. Vet Med Int [Internet] 2010 [cited 2010 Nov 6]; 2010. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20871827

  36. Rebrin I, Sohal RS. Pro-oxidant shift in glutathione redox state during aging. Adv Drug Deliv Rev [Internet] 2008 [cited 2010 Nov 6]; 60(13-14):1545-1552. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18652861

  37. Cornelli U. Antioxidant use in nutraceuticals. Clin Dermatol [Internet] 2009 [cited 2010 Nov 6]; 27(2):175-194. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19167999

  38. Moustafa MH, Sharma RK, Thornton J, Mascha E, Abdel-Hafez MA, Thomas AJ, Jr., Agarwal A. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod [Internet] 2004 [cited 2010 Nov 6]; 19(1):129-138. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14688171




2020     |     www.medigraphic.com