Méndez-Pérez C, Rebollar-Téllez EA

Language: Spanish
References: 285
Page: 7-21
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ABSTRACT

Introduction. Localised cutaneous leishmaniasis (LCL) is endemic in the Peninsula of Yucatan. Leishmania mexicana is transmitted to humans by the infectious bite of the insect vector Lutzomyia olmeca olmeca and possibly by Lu. cruciata as well. Even though there are several previous studies on the ecology of both phlebotomid sandflies, potential genetic variations have not been determined for geographically separated sandfly populations
Objective. The main objective of this study was to evaluate the existence of variability of morphological characters among populations, and then to evaluate whether or not populations could be distinguished from each other using discriminant analyses
Materials and Methods. Specimens of Lu. olmeca olmeca and Lu. cruciata were collected in distinct locations of the southern states of Tabasco, Campeche, Yucatán y Quintana Roo. Thirty one morphological characteristics were measured using a micrometric for every specimen, using consistent techniques for preservation and slide mounting for all samples. Following univariant analyses, we then selected only the statistically significant morphological characteristics for a multivariant analysis to identify group characteristics.
Results. For Lu. olmeca olmeca, univariant analysis found that 26 out of 31 characteristics were statistically significant, whereas for Lu. cruciata, 16 out of 31 characteristics were significant. Subsequent multivariate analyses showed that Lu. olmeca olmeca from Bechanchén were statistically different (discriminant) from the rest of the populations. In the case of Lu. cruciata, the populations of Dos Naciones and La Libertad were statistically different (discriminant) from the rest of populations studied.
Conclusions. Geographic variability was found among the morphological characteristics of the studied populations at both individual levels using univariant analysis, and for several characteristics considered simultaneously via multivariant analysis. More studies are required to confirm the levels of heterogeneity and genetic isolation among populations of the two studied sandfly species.

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251. Coquillett DW. Discovery of blood-sucking Psychodidae in America. Entomol. News 1907; 18:101-2.

252. Vargas L, Díaz-Nájera A. Lista de flebótomos mexicanos y su distribución geográfica (Diptera: Psychodidae). Rev Inst Salub Enfer Trop Méx 1953; 13:309-14.

253. Fairchild GB, Hertig M. Geographic distribution of the Phlebotomus sandflies of Central America (Diptera: Psychodidae). Ann Entomol Soc Am 1959; 52:121-4.

254. Ibáñez-Bernal S. Phlebotominae (Diptera: Psychodidae) de México. l. Brumptomyia França y Parrot; Lutzomyia França, las especies de L. (Lutzomyia) Franca y del grupo Verrucarum. Folia Entomol Mex 1999; 107:61-116.

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256. Rebollar-Téllez EA, Orilla-Moguel H, Dzul-Manzanilla FA, Che-Mendoza A, Manrique-Saide P, Zapata-Peniche A. An update on the phlebotomid sandfl y (Diptera: Phlebotomidae) fauna of Yucatan, Mexico. Entomol News. 2006; 117:21-4.

257. Ibáñez-Bernal S, Hernández-Xoliot RA, Mendoza F. Collections of Bruchomyiinae and Phlebotominae (Diptera: Psychodidae) from north-cental portion of the State of Veracruz, México, with the description of a new species. Zootaxa 2006; 1270:19-33.

258. Lane RP, Ready PD. Multivariate discrimination between Lutzomyia wellcomei. A vector of mucocutaneous leishmaniasis, and Lu. complexus (Diptera: Phlebotominae). Ann Trop Med Parasitol 1985; 79:469-72.

259. Añez N, Valente DT, Cazorla D, Quicke DJ, Feliciangeli M. D. Multivariate analysis to discriminate species of phlebotomine sandflies (Diptera: Psychodidae): Lutzomyia townsendi, L. spinicrassa, and L. youngi. J Med Entomol 1997; 34:312-6.

260. Bustamante DC, Monroy C, Menes M, Rodas A, Salazar-Schettino PM, Rojas G, Pinto N, Guhl F, Dujardin JP. Metric variation among populations of the Chagas vector Triatoma dimidiata (Hemiptera: Reduviidae: Triatominae) and related species. J Med Entomol 2004; 41:296-301.

261. Catala SS, Maida DM, Caro-Riaño H, Jaramillo N, Moreno J. Changes associated with laboratory rearing in antennal sensilla patterns of Triatoma infestans, Rhodnius prolixus, Rhodnius pallescens (Hemiptera: Reduviidae). Mem Inst Oswaldo Cruz 2004; 99:25-30.

262. Rangel EF, Lainson R, Souza AA, Ready P, Azevedo ACR. Variation between geographical populations of Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho, 1939) sensu lato. (Dipera, Psychodidae, Phlebotominae). Mem Inst Oswaldo Cruz. 1996; 91:43-50.

263. Martínez-Ibarra JA., Bárcenas-Ortega NM, Romero-Napoles J, Nogueda-Torres B, Rodríguez-López MH. Diferencia métricas entre poblaciones de Meccus longipennis (Usinger) (Hemiptera: Reduviidae) en el occidente de México. Folia Entomol Mex 2006; 45:83-90.

264. Cruz-Ruiz AL, Garcia-Rejón J, Manrique-Saide P, Perez-Mutul J. Taxonomical Identification of anthropophilic species of Lutzomyia in Quintana Roo, Peninsula de Yucatan, Mexico. Rev Bioméd 1994; 5:127-131.

265. Ibáñez-Bernal S. Phlebotominae (Díptera: Psychodidae) de México. II. Las especies de Ibáñez-Bernal S. Lutzomyia (Coromyia) Barretto, del grupo Delpozoi de Lutzomyia (Dampfomyia) Addis. Folia Entomol Mex 2001; 40:17-4641.

266. Ibáñez-Bernal S. Phlebotominae (Díptera: Psychodidae) de México. IV. Las especies de Lutzomyia (Psychodopygus) Mangabeira, L. (Micropygomyia) Barretto, Lutzomyia grupo Oswaldoi, L. (Helcocyrtomyia) Barretto y especies sin agrupar. Folia Entomol Mex 2003; 42:109-52.

267. Shannon R. Methods for collecting and feeding mosquitoes in jungle yellow fever studies. Am J Trop Med Hyg 1939; 19:131-40.

268. Pérez E, Villaseca P, Campos M, Guerra H. Técnicas para colectar “titiras” (Lutzomyia spp., Diptera: Psychodidae) en ambientes altoandinos peruanos. Rev Peru Entomol 1987; 30:77-80.

269. Disney RHL. A trap for phlebotomine sandflies attracted to rats. Bull Entomol Res 1966; 56: 445-451.

270. Young DG. A Revision of the Blood Sucking Psychodidae Flies of Colombia (Diptera: Phlebotominae and Sycoracinae). Gainesville, FL. University of Florida. 1979.

271. Sokal RR, Rolhf FJ. Biometry: the Principles and Practice of Statistics in Biological Research. Third edition. New York. 1995

272. Lehmann P, Ordoñez R, Ojeda-Baranda R, Mendez de Lira J, Hidalgo-Sosa L, Monroy C, Ramsey JM. Morphometric analysis of Triatoma dimidiata populations (Reduviidae: Triatominae) from Mexico and Northern Guatemala. Mem Inst Oswaldo Cruz. 2005; 100:477-82.

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