Rodríguez-Ulloa C, Rivera-Jacinto M

Language: Spanish
References: 15
Page: 516-519
PDF size: 91.57 Kb.

ABSTRACT

Objetive. To compare a commercial coproantigen ELISA kit and the technique of spontaneous sedimentation in tube (TSET) for the diagnosis of Giardia lamblia in fecal specimens from children in a Peruvian endemic area. Material and methods. 174 fecal samples were analyzed by TSET and 2^nd Generation Giardia ELISA kit. Results. 51 samples were positive by ELISA and 49 by TSET. Conclusions. The ELISA was highly sensitive and specific, simple and fast. However, the very good agreement, high precision, low cost and ability to detect other intestinal parasites makes use of TSET recommended for laboratory diagnosis in endemic areas of Peru.

REFERENCES

1. World Health Organization. The World Health Report 1996. Fighting Disease Fostering Development. Geneva: World Health Organization, 1996.

2. Alarcón J, Castro C, Murillo J. Prevalencia de giardiasis en encuestas parasitológicas publicadas en la literatura peruana, 1943-1990. Rev Per Epid 1993; 6: 5-17.

3. Botero D, Restrepo M. Parasitosis humanas. 4º ed. Bogotá: Corporación para Investigaciones Biológicas, 2006.

4. Núñez FA. Giardia lamblia. En: Llop A, Valdés-Dapena M, Zuazo JL, eds. Microbiología y Parasitología Médicas. Vol.3. La Habana: Editorial Ciencias Médicas, 2001:31-38.

5. Casanova RT, Ramos MC. Técnicas de diagnóstico de enfermedades causadas por enteroparásitos. Diagnóstico 2000;39:197-198.

6. Pajuelo-Camacho G, Luján-Roca D, Paredes-Pérez B, Tello-Casanova R. Aplicación de la técnica de sedimentación espontánea en tubo en el diagnóstico de parásitos intestinales. Ver Biomed 2006; 17: 96-101.

7. Terashima A, Marcos L, Maco V, Canales M, Samalvides F, Tello R. Técnica de sedimentación en tubo de alta sensibilidad para el diagnóstico de parásitos intestinales. Rev gastroenterol Perú 2009; 29: 305-310.

8. Chakarova B. Comparative evaluation of the diagnostic methods for detection of Giardia intestinalis in human fecal samples. TJS 2010;8:174-179.

9. Vidal AMB, Catapani WR. Enzyme-linked immunosorbent assay (ELISA) immunoassaying versus microscopy: advantages and drawbacks for diagnosing giardiasis. São Paulo Med J 2005; 123:282-285.

10. Weitzel T, Dittrich S, Mohl S, Adusu E, Jelinek T. Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samples. Clin Microbiol Infect 2006; 12: 656-659.

11. Vidal MF, Gilman RH, Ungar BLP, Verastegui MR, Benel AC, Marquis G, et al. Detection of Giardia lamblia antigen in children living in a peruvian periurban shantytown. J Clin Microbiol 1991;29:636-637.

12. Al-Saeed AT, Issa SH. Detection of Giardia lamblia antigen in stool specimens using enzyme-linked immunosorbent assay. EMHJ 2010; 16: 362-364.

13. Duque-Beltrán S, Nicholls-Orejuela RS, Arévalo-Jamaica A, Guerrero- Lozano R, Montenegro S, James MA. Detection of Giardia duodenalis antigen in human fecal eluates by enzyme-linked immunosorbent assay using polyclonal antibodies. Mem Inst Oswaldo Cruz, Rio de Janeiro 2002; 97: 1165-1168.

14. Corripio IF, Cisneros MJG, Ormaechea TG. Diagnóstico de las parasitosis intestinales mediante detección de coproantígenos. Enferm Infecc Microbiol Clin 2010; 28:33-39.

15. Rosoff JD, Sanders CA, Sonnad SS, De Lay PR, Hadley WK, Vincenzi FF, et al. Stool diagnosis of giardiasis using a commercially available enzyme immunoassay to detect Giardia-specific Antigen 65 (GSA 65). J Clin Microbiol 1989; 27:1997.