2011, Number 3
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Rev Biomed 2011; 22 (3)
Detection of adenoviruses from bronchoalveolar lavage fluid in HIV- infected Cuban patients
González-Muñoz G, Poumier O, Piñón A, Savón C, Valdés O, Acosta B, Goyenechea Á, Oropesa S, González-Báez G
Language: Spanish
References: 25
Page: 95-101
PDF size: 156.41 Kb.
ABSTRACT
Introduction. The significance of AdV infection in immunocompromised patients has been firmly established. These infections have the potential to cause disseminated disease with high mortality rates, and the clinical management of infected patients is a major problem.
Objective. To research the role of Adenovirus in Respiratory Infections in HIV-infected Cuban patients, and to characterize the isolates obtained in species and serotypes using Molecular Biology techniques.
Materials and Methods. 21 samples of bronchoalveolar lavage fluid from HIV-positive patients with respiratory symptoms of prolonged evolution with uncertain etiology, in which there was a predominance of chronic pneumonia were researched. In spite of antibiotic therapy, their problems remained unresolved. The initial diagnoses were performed by PCR and viral cultures. The identification and typing of AdV isolates were achieved by PCR. Other respiratory pathogens, such as bacteria and fungi were also investigated.
Results. In 10 (48%) out of the 21 samples the presence of AdV was detected, and the virus was isolated in 7 of them (70%). Pneumonia was the predominant clinical manifestation (50 % of the cases).The AdV found belonged to species B (28%) and C (71%), type 3 and types 1 and 2, respectively. In 80% of the cases, only AdV was found, and in 20% AdV was found together with bacteria.
Conclusions. Adenoviruses were identified as causative for respiratory infections in HIV-positive patients, particularly the B and C species. For that reason, including them in diagnostic testing would be useful in treating and managing HIV patients with respiratory infections. The combination of classical and molecular techniques proved very effective in diagnosing, identifying and characterizing adenoviruses.
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