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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2011, Number 3

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Rev Biomed 2011; 22 (3)

Differential agglutination of promastigotes of Leishmania infantum Venezuelan strain with lysates from midgut of Lutzomyia evansi and Lutzomyia longipalpis (Diptera:Psychodidae)

Oviedo M, Bastidas G, Sandoval CM, Vivenes A, Bendezu H

Language: Spanish
References: 45
Page: 85-93
PDF size: 158.93 Kb.


ABSTRACT

Introduction. The parasite Leishmania sp. must adapt the physiological conditions of the digestive tract of the insect that transmits, to ensure their survival and subsequent transmission to the vertebrate host. Leishmania-vector interaction depends on several factors, including the agglutinins of the digestive tract of sandflies, involved along with lipophosphoglycan molecules of the parasite surface, the adhesion of the latter, the intestinal epithelium of the insect. Therefore, both molecules are involved in susceptibility of the sandfly species to infection.
Objective. To measure and compare the agglutination titer between midgut lysates L. longipalpis and L. evansi wild and colony with L. infantum promastigotes.
Materials and Methods. The methodology consisted in the evaluation of three groups of lysates of midgut Lutzomyia females, one of females captured in the wild, and two other colony, a females taken 48-hours blood ingest and the other females maintained with a sugary diet for 48 hours.
Results. Find different titles of agglutination between promastigotes of different days of colony of L. infantum and lysates of midgut of L. evansi and L. longipalpis. The only species that register agglutination in dilutions of 1:64 is L. longipalpis.
Conclusion. This study revealed the presence of agglutinins against promastigotes of L. infantum in populations of wild habitat and of colony of L. evansi and L. longipalpis, but higher in the latter species of phlebotomies. Titles of agglutination increase as it does, the time of cultivation of the Leishmania promastigotes.


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