2011, Number 4
<< Back Next >>
salud publica mex 2011; 53 (4)
Comparative study of a rapid testing with real time RT-PCR for diagnosis of influenza AH1N1 2009
Castro-Cárdenas LA, Llaca-Díaz JM, Pérez-Chávez F, Gómez-Espinel IA, Flores-Aréchiga A
Language: Spanish
References: 14
Page: 329-333
PDF size: 255.37 Kb.
ABSTRACT
Objective. Compare QuickVue Influenza A+B test with real-time RT-PCR for the diagnosis of influenza AH1N1 2009.
Material and Methods. Retrospective-comparative study of 135 respiratory specimens from individuals with symptoms of influenza processed from May 2009 to October 2010. The above mentioned tests were performed simultaneously. For statistic analysis the software of Confidence Interval Analysis 2000 was used.
Results. The parameters obtained were: sensitivity 62.96; specificity 94.44; negative predictive value 62.9; positive predictive value 94.44; positive likelihood ratio 11.33; negative likelihood ratio 0.39. Confidence intervals to 95, were calculated to all of the above data.
Discussion. The test QuickVue Influenza A+B is a rapid, simple test, with lower cost than real-time RT-PCR useful for identifying the type of virus outbreaks of influenza in a given population. It correlates well with more specific test and similar reports.
REFERENCES
Ison M, Lee N. Influenza 2010-2011: Lessons from the 2009 pandemic. Cleveland Clinic Journal of Medicine 2010; 77: (11) 812-820.
Ramos-Jiménez J. Infectología clínica. México: El Manual Moderno, 2008: 313-323.
Takahashi H, Otsuka Y, Patterson B. Diagnostic test for influenza and other respiratory viruses: determining performance specifications based on clinical setting. J Infect Chemother 2010; 16: 155-161.
Gavin PJ, Thomson RB. Review of rapid diagnostic test for Influenza. Clinical and Applied Inmunology Reviews 4: 2003; 151-172.
Quidel Corporation. [sitio de internet]. San Diego: QuickVue Influenza A+B test. [Consultado 2009 marzo 29]. Disponible en: http://www.quidel.com/products/product_detail.php?prod=101&group=1&show=spec
Centro para el Control y la Prevención de Enfermedades. [sitio de internet]. Atlanta: CDC protocol of realtime RTPCR for swine influenza A (H1N1). Última actualización 30 de abril de 2009. [Consultado 2009 abril 1]. Disponible en: http://www.who.int/csr/resources/publications/swineflu/CDCrealtimeRTPCRprotocol_20090428.pdf)
Altman D, Machin D, Bryant T, Gardner M. Statistics with confidence. 2a. edición. Bristol: BMJ, 2000.
Altman D, Bland J. Diagnostic test: sensitivity and specificity. BMJ 1994; 308:1552.
González J, Iglesias JM, Romero Y, Chávez C, Gay JG, Rivas R. Costoefectividad en la detección de influenza H1N1: datos clínicos versus pruebas rápidas. Rev Panam Salud Publica 2011;29 (1):1-7.
Figueroa C, Núñez L, Aranda D, Gómez S, Osorio E, Vargas H. Análisis de concordancia de cuatro pruebas rápidas para la detección de influenza A en Bogotá. Univ Med Bogotá (Colombia) 2009;50 (4): 444-451.
Ginocchio CC, Zhang F, Manji R, Arora S, Bornfreund M, Falk L, et al. Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak. Journal Clinical Virology 2009;45: 191-195.
Delgado M, Llorca J. Estudio de las pruebas diagnósticas. En: Piédrola-Gil. Medicina preventiva y salud pública 10ª ed. Barcelona: MASSON, 2001:145-155.
Atmar R, Baxter B, Domínguez E, Taber L. Comparison of reverse transcription-PCR with Tissue culture and other rapid diagnostic assays for detection of type A Influenza virus. J Clin Microbiol 1996; 2604-2606.
Centro Nacional de Vigilancia Epidemiológica y Control de Enfermedades [sitio de internet]. México: Influenza “En Chihuahua”. Última actualización 30 de marzo de 2011. [Consultado 2011 abril 2]. Disponible en: http://www.cenavece.salud.gob.mx/interior/influenza2011.html