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2005, Number 3

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Rev Mex Patol Clin Med Lab 2005; 52 (3)

In vitro study of lead diminution in erythrocytes in presence of glucose

Zecua HG, Ochoa RA, Ibarra VE

Language: Spanish
References: 23
Page: 59-63
PDF size: 48.40 Kb.


ABSTRACT

Lead is one of the most abundant elements in nature because it comes from many natural and anthropogenic sources. Most of the population of urban areas breathes an average of 40 mg of lead everyday. From that average, just 15 mg are distributed in the body among the soft tissues, bones, etc. These lead may pass trough the placenta or the hematoencefalic barrier causing a disease called saturnism. Lead may inhibit or diminish the functionality of certain enzymes such as delta aminolevulic sintetase (that inhibits the production of HEM groups. For this investigation it was run an experiment using human erythrocytes in vitro in order to see if the level of glucose in the body has any relationship with the absorbance of lead by the erythrocytes. For this experiment the erythrocytes were exposed to lead nitrate for 120 minutes. Several substances were added to the above mixture to analyze the final level of lead absorbance; the substances used were penicillamine, EDTA, cistein and glucose. The first three did diminish the lead’s absorbance; glucose also had a similar effect but in a less percentage. This effect of glucose is due to an indirect process rather than a direct one. The metabolism of glucose in the erythrocytes is made through the cycle of monophosphate pentose and the presence of reduced glucose captures some lead because of the presence of sulfhydryl groups (same case as cystein). All lead’s measures were made using ditozone with carbon tetrachloride.


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