1999, Number 3
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Cir Plast 1999; 9 (3)
Therapy of full-thickness burns with meshed autografts covered with frozen human allograft epidermal cultures. A case report
Sosa-Serrano AFJ, Álvarez-Díaz CJ, Cuenca-Pardo J, Juárez-Aguilar E, Kuri-Harcuc W
Language: Spanish
References: 16
Page: 126-129
PDF size: 156.42 Kb.
ABSTRACT
It has been demonstrated that cultured human allogeneic epidermal sheets promote faster epithelization of burns. Treatment of a 3 year-old girl with full-thickness burns over 46 percent of her body with 6 to 1 meshed autografts and donor sites, covered with frozen allografts epidermal cultures is discussed. With this combined therapy total epithelization of the wounds healed 29 days after the beginning of the treatment. The use of cultured allografts combined with conventional therapy reduces the time required to complete wound re-epithelization and therefore they reduce the time patients have to stay in hospital. Moreover, prompt availability and the ease of use of these frozen cultures make this resource an excellent complement in the burn patient therapy.
REFERENCES
Kreis RW, Mackie DP, Hermans RR, Vloemans AR. Expansion techniques for skin grafts: Comparison between mesh and Meek island (sandwich-) grafts. Burns 1994; 20 sppl 1: S39.
Vandeput J, Nelissen M, Tanner JC, Boswick J. A review of skin meshers. Burns 1995; 21: 364.
Alexander JW, MacMillan BF, Law E et al. Treatment of burn with widely meshed skin autograft and meshed skin allograft overlay. J Trauma 1981; 21: 433.
Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell 1975; 6: 331.
Green H, Kehinde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 1979; 76: 5665.
Bolívar-Flores J, Poumian E, Marsch-Moreno M, Montes de Oca G, Kuri-Harcuch W. Use of cultured human epidermal keratinocytes for allografting burns and conditions for temporary banking of the cultured allografs. Burns 1990; 16: 3.
Nuñez-Gutierrez H, Castro-Muñozledo F, Kuri-Harcuch W. Combined use of allograft and autograft epidermal cultures in therapy of burns. Plast Reconstr Surg 1996; 98: 929.
Rivas-Torres M, Amato D, Arámbula-Alvarez H, Kuri-Harcuch W. Controlled clinical study of skin donor site and deep partial thickness burns treated with cultured epidermal allografts. Plast Reconstr Surg 1996; 98: 279.
Arámbula-Alvarez H, Sierra-Martinez E, Gónzalez-Aguirre NE et al. Frozen human epidermal allogeneic cultures promote rapid healing of facial dermabrasion wounds. Dermatol Surg 1999; 25: 708.
Bolívar-Flores JY, Kuri-Harcuch W. Cryopreserved epidermal cultures promote healing of chronic and complicated leg ulcers. Dermatol Surg 1999; 25: 610.
Alvarez-Díaz C, Cuenca-Pardo J, Sosa-Serrano A, Juárez-Aguilar E, Kuri-Harcuch W. Controlled clinical study of deep partial thickness burns treated with frozen cultured human allogeneic epidermal sheets. J Burn Care Rehabil; En prensa.
Tamariz E, Castro-Muñozledo F, Tsutsumi V, Kuri-Harcuch W. Cultured sheets of human epidermal keratinocytes enhance healing of full-thickness wounds in mice. Cell Tissue Res 1999; 296: 575.
Nguyen TT, Gilpin DA, Meyer NA, Herndon DN. Current treatment of severely burned patients. Ann Surg 1996; 223: 14.
Bull JP, Fisher AJ. A study of mortality in a burns unit: A revised estimate. Ann Surg 1954; 139: 269.
Muller MJ, Herndon DN. The challenges of burns. Lancet 1994; 343: 2216.
Morrow SE, Smith DL, Cairns BA, Howell PD, Nakayama DK, Peterson HD. Etiology and outcome of pediatric burns. J Pediatr Surg 1996, 31: 329