2011, Number 2
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Vet Mex 2011; 42 (2)
Using a quick dye for the diagnosis of microsporidiosis
Rodríguez TLE, Rodríguez RHG, Valdez NY, Nevárez GAM, Ramírez RR
Language: English/Spanish
References: 19
Page: 179-185
PDF size: 332.17 Kb.
ABSTRACT
Cystic-like structures similar to xenomas containing ovoid or piriform organisms were observed in brain and kidney of a one-month-old rabbit. Different dyes such as H&E, PAS, toluidine blue and trichrome blue were tested. Trichrome blue yielded the best results in terms of specificity, resolution and confirmation of microsporidia spores. This dye is easily adaptable to paraffin-embedded samples by not requiring additional equipment; it is rapid, cheap and could be used as a confirmatory technique for other microsporidiosis.
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