Bouchan-Valencia P, González-Jiménez MA, Paredes-Vivas LY, Calixto-González R, Vázquez-Rodríguez S, Montaño-Rodríguez R, Estrada-Parra S, Cérbulo-Vázquez A

Language: Spanish
References: 15
Page: 228-235
PDF size: 316.97 Kb.

ABSTRACT

Human milk is not only a source of nutrients to the milk-suckle infant but also a supplier of humoral immunity. Using animal models and observation in immunodeficient humans, recent some reports show that not only antibodies but cells could be transfer from mother to fetus or newborns. Human colostrum contains about 5 x 10⁶leukocytes/mL being macrophages and polymorphonuclear leukocytes the most frequent population meanwhile T, B and NK lymphocytes just represent 10% of the population. We report the immunophenotype of mononuclear cells-isolated from seven colostrum samples. Using flow cytometry CD3⁺CD4⁺, CD3⁺CD8⁺, CD19⁺ or CD16⁺CD56+ that expressed CD25, CD28, CD45RA, CD45RO, CD69, CD80, CD86, CD94, CD152 or HLA-DR were quantified in colostrum from asymptomatic women. Our results showed the presence of T cells with a CD3⁺CD4⁺CD25⁺ phenotype up to 40% of the population, suggesting the presence of lymph ocytes with potential for regulatory function. Classic markers of cellular activation as CD45RO⁺, CD69⁺ or HLA-DRbright not appear to be largely represented. Our study shows for the first time the percentage of T and NK cells expressed in colostrums which are expressing CD80, CD86 or CD94.

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