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2010, Number 5

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Acta Pediatr Mex 2010; 31 (5)

Comparative study of cranial bone developed by ostegenesis in experimental model rabbits

Sesman-Bernal AL, Barceló-de la Isla R, Herrera-Rosas A, Espejo-PlascenciaJI, Avila-Figueroa L, Velasquillo-Martínez MC, León-Pérez JA

Language: Spanish
References: 41
Page: 206-216
PDF size: 3742.32 Kb.


ABSTRACT

Background: There is a continuing interest in developing materials as substitutes for bone grafts to repair full-thickness cranial defects, especially for correction of birth and post-trauma defects, where the main limitation lies in autologous graft coverage of the bone defects. There were intend to develop a unit where simultaneously verify the osteoconduction, the osteoinduction and osteogenesis for the repair of such defects.
Methods: We performed a craniotomy to 100 mm2 (10 x 10 mm) by means of an oscillating drill, taking care not to damage the dura or the brain. The osteogenic unit was then applied on the experimental lesion, following the application of a scaffold of acellular human dermis on the defect in contact with the dura, finally demineralized bone and autologous bone marrow are placed.
Results: Light microscopy analysis of histological parameters were compared between groups and there was a significant difference in the proportion of osteoid tissue (p ′0.05), immature trabecular (p ′0.05) and number of osteoblasts (p ′0.05). These variables showed an increase in animals killed at the second month of the study, while they decreased in animals slaughtered on the sixth postsurgical month.
Conclusions: The implanted experimental model fullfilled established the three osteogenic reconstruction biological factors: Osteinduction, osteoconduction and osteogenesis.


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