Garrido FGI, Cornejo CMÁ

Language: English/Spanish
References: 9
Page: 373-377
PDF size: 40.76 Kb.

ABSTRACT

A protocol for fast embedded paraffin by a microwave process for histological and clinical samples for light microscopy was developed. Tissue specimens, dog’s liver, polyp, skin and lung, cat’s brain, kidney, and rat’s ovary, uterine horn and artery were processed by using a standard household microwave oven (600 watts) at 75% power. This study was done using progressive alcohol solutions (dehydratation), bencene-etanol solutions (clearing) and paraffin for the infiltration. A group of specialists reviewed the material and photographs of the different organs processed with three different inclusion techniques. Results of the observation revealed: no difference between the histokinette (HK) and microwave oven. There were apparent morphological differences between the last two processes and the Robinson & Fayen technique (RF). This process could allow results to be reported within 30 min after the specimens were evaluated in the laboratory. The morphology for the microwave-processed samples was better than the one observed by the fast diagnostic process technique of RF, and no discerning differences with the routine histokinette method were found.

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