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Federación Mexicana de Ginecología y Obstetricia, A.C.

2010, Number 12

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Ginecol Obstet Mex 2010; 78 (12)

Effect of merocianina-540 in the fertilizing capacity of sperm correlated with the expression of 3-OH terminal fragments

Barroso-Villa G, Colin-Valenzuela A, Gómez-López N, Velázquez-Magaña M

Language: Spanish
References: 23
Page: 685-691
PDF size: 368.91 Kb.


ABSTRACT

Background: The packaging of heterochromatin during spermatogenesis has been correlated with the expression of residual apoptotic bodies (which stain with merocyanine A) that will impact on sperm function in the fertilization process; as well as the joint expression of the transmembrane translocation phosphatidyl serine and oligonucleosomes.
Objective: To evaluate the expression of bodies stained with merocyanine in the functional processes of sperm and their level of agreement with apoptotic Annexin V and TUNEL biomarkers.
Material and method: We performed a prospective, cross, including 11,000 cells belonging to semen samples from infertile men, were evaluated according to WHO criteria (1999), bounded by the lines of Tygerberg. The biomolecular transformation processing of the membrane and the expression of oligonucleosomes in the terminal cascade of apoptosis were quantified by cytometry flow, using an argon lasser as a reading source of 480 nm, discriminating the degree of cellularity, both negative and positive for each indicators.
Results: Because of the study design was found low quantification in semen parameters, motility, morphology and sperm concentration.The average expression of cells [DNA-PI(+) / dUTP-FITC(+)] (quantification of TUNEL) and [Annexin-V(+) / PI(-)] was 36.5 ± 17.4% and 31.2 ± 17.4%, respectively. By comparing the expression of TUNEL without the effect of M540 bodies (36.3 ± 1.7% vs. 36 ± 1.7%) a significantdifference was not determined.
Conclusions: This study shows that there is a remnant of the primary processes of spermiation, which can take an important role in apoptotic and functional processes of the sperm. However, its expression does not affect measurement of biomarkers of apoptosis seminal, whose determination changed the diagnosis and functional perception of reproductive parameters in the sperm.


REFERENCES

  1. Barroso G, Valdespin C, Vega E, Kershenovich R, Avila R, Avendano C et al. Developmental sperm contributions: fertilization and beyond. Fertil Steril 2009;92:835-48.

  2. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000;15:1338-44.

  3. Spano M, Bonde JP, Hjollund HI, Kolstad HA, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. Fertil Steril 2000;73:43-50.

  4. Weng SL, Taylor SL, Morshedi M, Schuffner A, Duran EH, Beebe S et al. Caspase activity and apoptotic markers in ejaculated human sperm. Mol Hum Reprod 2002;8:984-91.

  5. Mower DA, Jr., Peckham DW, Illera VA, Fishbaugh JK, Stunz LL, Ashman RF. Decreased membrane phospholipid packing and decreased cell size precede DNA cleavage in mature mouse B cell apoptosis. J Immunol 1994;152:4832-42.

  6. Rathi R, Colenbrander B, Bevers MM, Gadella BM. Evaluation of in vitro capacitation of stallion spermatozoa. Biol Reprod 2001;65:462-70.

  7. Oehninger S, Morshedi M, Weng SL, Taylor S, Duran H, BeebeS. Presence and significance of somatic cell apoptosis markers in human ejaculated spermatozoa. Reprod Biomed Online 2003;7:469-76.

  8. Barroso G, Taylor S, Morshedi M, Manzur F, Gavino F, Oehninger S. Mitochondrial membrane potential integrity and plasma membrane translocation of phosphatidylserine as early apoptotic markers: a comparison of two different sperm subpopulations. Fertil Steril 2006;85:149-54.

  9. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000;15:1338-44.

  10. Muratori M, Marchiani S, Maggi M, Forti G, Baldi E. Origin and biological significance of DNA fragmentation in human spermatozoa. Front Biosci 2006;11:1491-9.

  11. Moskovtsev SI, Willis J, White J, Mullen JB. Sperm DNA damage: correlation to severity of semen abnormalities. Urology 2009;74:789-93.

  12. Marchiani S, Tamburrino L, Maoggi A, Vannelli GB, Forti G, Baldi E et al. Characterization of M540 bodies in human semen: evidence that they are apoptotic bodies. Mol Hum Reprod 2007;13:621-31.

  13. Henkel R, Maass G, Jung A, Haidl G, Schill WB, Schuppe HC. Age-related changes in seminal polymorphonuclear elastase in men with asymptomatic inflammation of the genital tract. Asian J Androl 2007;9:299-304.

  14. Muratori M, Marchiani S, Criscuoli L, Fuzzi B, Tamburino L, Dabizzi S et al. Biological meaning of ubiquitination and DNA fragmentation in human spermatozoa. Soc Reprod Fertil Suppl 2007;63:153-8.

  15. Oldereid NB, Angelis PD, Wiger R, Clausen OP. Expression of Bcl-2 family proteins and spontaneous apoptosis in normal human testis. Mol Hum Reprod 2001;7:403-8.

  16. Muratori M, Porazzi I, Luconi M, Marchiani S, Forti G, Baldi E. AnnexinV binding and merocyanine staining fail to detect human sperm capacitation. J Androl 2004;25:797-810.

  17. Seli E, Sakkas D. Spermatozoal nuclear determinants of reproductive outcome: implications for ART. Hum Reprod Update 2005;11:337-49.

  18. Marchiani S, Tamburrino L, Forti G, Baldi E, Muratori M. M540 bodies and their impact on flow cytometric analyses of human spermatozoa. Soc Reprod Fertil Suppl 2007;65:509-14.

  19. World Health Organization. WHO Laboratory Manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge University Press, 1999.

  20. Durakbasi-Dursun HG, Zamani AG, Kutlu R, Gorkemli H, Bahce M, Acar A. A new approach to chromosomal abnormalities in sperm from patients with oligoasthenoteratozoospermia: detection of double aneuploidy in addition to single aneuploidy and diploidy by five-color fluorescence in situ hybridization using one probe set. Fertil Steril 2008;89:1709-17.

  21. Duran EH, Morshedi M, Taylor S, Oehninger S. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod 2002;17:3122-8.

  22. Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi PG, Bianchi U. Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod 1999;4:31-7.

  23. Gandini L, Lombardo F, Paoli D, Caponecchia L, Familiari G, Verlengia C et al. Study of apoptotic DNA fragmentation in human spermatozoa. Hum Reprod 2000;15:830-9.




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