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Federación Mexicana de Ginecología y Obstetricia, A.C.

2010, Number 12

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Ginecol Obstet Mex 2010; 78 (12)

Impact of male aging in the functional capacity of sperm through the expression of phosphatidyl serine and oligonucleomas

Colin-Valenzuela A, Gómez-López N, Ávila-Lombardo R, Barroso-Villa G

Language: Spanish
References: 43
Page: 669-676
PDF size: 360.88 Kb.


ABSTRACT

Background: biomolecular processes associated with aging and programmed cell death during spermatogenesis are well known, but not its biological significance in ejaculated sperm, because it ignores the behavior of these apoptotic markers in relation to the age of man.
Objective: To evaluate the effect of aging on the functional capacity of sperm and their relationship to programmed cell death processes.
Material and methods: Prospective, cross-sectional and analytical performed with semen samples from 25 healthy subjects 20 to 70 years old, were divided into two groups [(A: under 40 years) and (B: over 40 years age)]. Semen parameters were evaluated WHO (1999) and transformation processes biomolecular membrane and the expression of oligonucleosomes in the terminal cascade of apoptosis. Were measured by flow cytometry with an argon laser as a source of reading at 480 nm, the degree of cellularity discriminate negative and positive for each of the indicators.
Results: The percentage of live cells with phosphatidylserine translocation in the membrane (annexin-V / PI) was significantly higher in men older than 40 years (p ‹ 0.05). These findings are enriched with a significant positive correlation (r = 0.50, P ‹ 0.008) between early biomarker and age of the subjects. With regard to DNA fragmentation, although no statistically significant differences were found, it is aclear trend of increase as older the subjects (r = 0.51).
Conclusions: The increasing age of the man is associated with increased expression of apoptosis, as demonstrated by the increased expression of phosphatidylserine translocation at the Membrane. Thus, this study confirms that the subject’s age is associated with a decline in some of the seminal parameters.


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