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2000, Number 4

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Vet Mex 2000; 31 (4)

Comparison between two different antibody sources for the development of radioimmunoassays to measure plasmatic levels of testosterone

Mórtola E, Endo Y, Risso M, Gimeno E

Language: English/Spanish
References: 42
Page: 271-277
PDF size: 67.26 Kb.


ABSTRACT

En el presente trabajo, se examinan y comparan los efectos a largo plazo de dos antioxidantes: N-acetilcisteína (NAC) y ácido ascórbico (AA), con dos agentes inmunosupresores: Ciclosporina A (CsA) y tacrolimus (también conocido como FK506) sobre la replicación viral y la apoptosis en cultivos de una línea celular de fibroblastos felinos crónicamente infectada con el virus de la inmunodeficiencia felina (VIF). En las células tratadas con NAC o AA se detectó una inhibición significativa de la replicación viral y la apoptosis, luego del agregado del factor de necrosis tumoral-alfa (TNF-a). Cuando las células fueron tratadas con los agentes inmunosupresores CsA y FK506, la replicación viral y la apoptosis descendieron en forma similar a lo hallado con el uso de los antioxidantes. La inhibición del virus VIF en los cultivos tratados con cualquiera de los fármacos utilizados, persistió durante el tiempo que duró la suplementación de las mismas. Sobre la base de estos resultados in vitro, que muestran la eficacia de cada componente sobre la replicación viral y la apoptosis, podría sugerirse que el mejor camino para retrasar la aparición del síndrome de inmunodeficiencia adquirida es a través del uso inteligente de una combinación de terapias.


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