Lobato-García BR, Rivera-Ochoa N, Rodríguez-Sánchez VM, Rodríguez-Morales P, Callejas-Morales F, Cervantes-Toache MC, Morales-Fierro C, Aguilar-Roustand HA, Arenas MDG, Moreno-Herrera J, Barrientos-Sánchez CD, Bueno-García S, Carmona-Cortés DA, Lozano BJE, Rodríguez-Trujillo L, Ceballos-Grajales N, Lara-Lince LC, León–Palmeros GA, Mendoza TDY, González HSL, Juárez AE

Language: Spanish
References: 10
Page: 5-12
PDF size: 340.30 Kb.

ABSTRACT

INTRODUCTION: Teaching of biomedical sciences in the new educative paradigm in the Universidad Veracruzana must include laboratory sessions that promotes scientific interest and curiosity in the students. OBJECTIVE: The present work describe an exercise of educational innovation through the optimization of two immunization protocols used in the teaching of thebasis of humoral immune response throughout the production of polyclonal antibodies against human serum (Hs) and egg albumin (Ea) on adult rabbits. MATERIALS AND METHODS: It were detected improvement points in the protocols which could be approached for the students. Specifically, it was proposed to improve the route of antigen administration and the route of sample blood collection. Antigens were administratedsubcutaneously in several doses in the chest and the lumbar zone. Each antigen doses were inoculated on these regions in different points (eight maximum) in order to comprise a extense absorption area. Animals were blooded twenty days after the initial immunization through punction of an artery or vein ear. Antibodies detection in the immune sera was revealed in vitro through a precipitation reaction as result of antibody-antigen interaction. The antibodies produced were characterized by double immunodifusion (DID), radial immunodifusion (RID) and immunoelectrophoresis (IE).

REFERENCES

1. Huberman AM. Cómo se realizan los cambios en la educación: una contribución al estudio de la innovación. París: UNESCO-OIE; 1973.

2. Havelock RG. y Huberman AM. Innovación y problemas de la educación. Teoría y realidad en los países en desarrollo, Ginebra: UNESCO-OIE; 1980.

3. Carpenter PL. Inmunología y Serología 2a. ed. México: La Prensa Médica Mexicana; 1982.

4. Douglas FA, and Suckow MA. The Laboratory Rabbit. Boca Raton (USA): CRC Press; 1997.

5. Marton B y cols. Extracción de sangre de mamíferos y aves de laboratorio. Primer informe del grupo de trabajo BVA/FRAME/ RSPCA/UFAW sobre refinamiento. Anim Lab 1998; 27: 1-22.

6. University of Kentucky Medical Center. Standard operating procedure IACUC 103. Rabbit blood collection protocol. 98 Jun [citado en 2003]; 1 (1): [24 screens]. Disponible en: http://www.mc.uky.edu/dlar/ resources/sop/rabbleed.html

7. IACUC. Antibody Production in Rabbits. [citado en 2003]; 1 (1): [24 screens]. Disponible en: http://iacuc.yale.edu/policies/rabbits.html

8. Bledding and immunization techniques for polyclonal antibody production. [Citado en 2003]. Disponible en: http://www.molbio. princeton.edu/facility/restricted/viv_restricted/rabbitbleed.html.

9. SUNY Upstate Medical University. Guidelines for the production of policlonal and monoclonal antibodies in rodents and rabbits. 2001 Oct [citado en 2003]; 1 (1): [24 screens]. Disponible en: http://www. upstate.edu/dlar/Antibody%20Production.pdf

10. University of Florida. Guidelines for the production of policlonal and monoclonal antibodies in rodents and rabbits. [citado en 2003]; 1 (1): [24 screens]. Disponible en: http://www.health.ufl.edu/acs/infopi/ antibodis.html Optimización de protocolos