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Órgano Oficial del Instituto de Ciencias de la Salud, Hospital Escuela y Facultad de Medicina-Xalapa

2009, Number 1

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Rev Med UV 2009; 9 (1)

Evolution of culturemedium in the study of neural stem cells

Hernández GEA, Juárez AE

Language: Spanish
References: 71
Page: 16-23
PDF size: 233.05 Kb.


ABSTRACT

Since its development at beginnings of 20th Century, cell culture media has played a relevant roll in the in vitro cell study. In this new century, media cell culture has been improved, knowing their exact formulation, without animal sera supplementation. Due to the absence of animal serum in these media, they are called “serum free medium” (SFM). Nowadays, SFM are essential for the study of several cellular types, supporting cellular proliferation and diff erentiation. Atier a series of changes throughout years, SFM have been used for study of the so-called “stem cells”. Specifically, SFM has played an important roll in the study of the neural stem cells, whose discovery in the adult destroyed the dogma of the static brain. The discovery of neural stem cells have opened the possibility of cell therapies development for several neurodegenerative diseases like Alzheimer and Parkinson. In the cell therapies development, the SFM play a relevant roll. In this way, this paper will review the scientific literature about SFM and its possible improvement for the isolation and propagation of neural stem cells


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