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2009, Number 3

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Rev Mex Cir Pediatr 2009; 16 (3)

Urethroplasty with frozen cultured sheets of human epidermal keratinocytes Experimental study in dogs

Garibay-González F, Arroyo LR, Navarrete-Arellano M

Language: Spanish
References: 20
Page: 126-133
PDF size: 293.56 Kb.


ABSTRACT

Introduction: Hypospadias is one of the most common congenital malformations. (30 a 39.7/10,000 newborns). Urethroplasty for correction of hypospadias is still a challenge. No matter surgical technique, postoperatory complications (stenosis, diverticulum and fistula) are frequent. Urethrocutaneous fistula is the most common.
Potentially, urethroplasty postoperatory complications could be lower using frozen epidermal sheets.
Objetive: To show that frozen cultured sheets of human epidermal keratinocytes could decrease urethroplasty postoperatory complications in an experimental dog model.
Material and Methods: Experimental, comparative study in 20 dogs. Control group: Snodgrass urethroplasty technique (A, n=10) and experimental group (B, n= 10), urethroplasty with cover of human epidermal keratinocytes. Variables were: inflammation, fibrosis, ephitelization and complications.
Results: There were 3 fistulas in group A (30%) and no one in group B. Stenosis was present in one dog of group A (10%) and there were no diverticulum in both groups. Group B showed less number of total complications (p=0.0253). We could not probe any difference in inflammation, fibrosis and ephitelization (p› 0.05).
Conclusions: There were no postoperatory complications with the use of frozen cultured sheets of human epidermal keratinocytes; its use in urethroplasty technique seems to be safe in the studied animal group.


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