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Órgano Oficial del Instituto Nacional de Pediatría

2009, Number 5

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Acta Pediatr Mex 2009; 30 (5)

Tandem mass spectrometry: a new technique for metabolome study in pediatrics

Fernández-Lainez C, Vela-Amieva M, Ibarra-González I

Language: Spanish
References: 35
Page: 258-263
PDF size: 234.66 Kb.


ABSTRACT

Recent advances in technology have brought about a revolution in the manner in which biological systems are visualized and queried. Advances in mass spectrometry (MS) have made possible the analysis of cellular proteins and metabolites (proteome and metabolome respectively) on a scale previously unimaginable.
The metabolome is defined as the quantitative collection of small molecular weight compounds (metabolites) present in cells, tissues or organisms which are the final downstream products of metabolic reactions. Quantitative and qualitative measurements of metabolites provide a broad view of the biochemical status of an organism.
MS is an analytical technique which allows separation, identification and simultaneous quantification of several metabolites, in complex biological matrices, in a very short period of time. Initially, it was created for research purposes and at the present time their accessibility has opened the path for the development of various applications such as analysis of several body compounds, the environment, drugs, food, toxicology and forensics.
Screening of the neonates is the most common application of MS; its fast expansion has set standards for new applications in the metabolomic area and represents a model for future applications such as screening for different diseases of newborns, pediatric and adult patients. The objective of the present work is to explain the technical basis of the MS, to mention some of its clinical applications which have converted it into the main tool for the development of metabolomics and to approach different pediatric problems.


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