Flores-Herrera H, Maida-Claros R, Solís-Herrera H, Illescas-Medrano E, Zavala-Díaz de la Serna FJ

Language: Spanish
References: 38
Page: 148-155
PDF size: 685.06 Kb.

ABSTRACT

Neonatal sepsis is a worldwide public health problem in many newborns. Its incidence is estimated at 8 of 1000 alive births; however the risk of morbility and mortality reaches 50 %. Neonatal sepsis presents with signs and symptoms of infections; its diagnosis is confirmed by blood culture. It may be present in one of two forms: In one group there are no signs or symptoms initially; they appear several days later which hinders and/or delays medical treatment. In another group there are signs and symptoms, while blood cultures are negative. It is estimated that 15% of all newborns with neonatal sepsis are treated with a variety of broad-spectrum antibiotics even without identification of a causal agent. Recently, molecular techniques such as the polymerase chain reaction (PCR) and the sequencing by identification of bacteriae associated with the neonatal sepsis. The main goal of this review is to present the basis of the PCR in combination with the denaturing gradient gel electrophoresis (DGGE) and the sequencing in cases suspected of the sepsis neonatal.

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