medigraphic.com

2010, Number 3

<< Back Next >>

Vet Mex 2010; 41 (3)

Morphometric characteristics of female reproductive organs of New Zealand rabbits with different body weight in peripuberal period of transition

Aragón HJ, Suárez SJ, Pérez-Martínez M

Language: English/Spanish
References: 16
Page: 211-218
PDF size: 227.77 Kb.


ABSTRACT

Morphometric characteristics were determined from female reproductive organs of New Zealand rabbits with different body weight in order to understand the anatomical changes of the various organs of the reproductive tract of this breed in the peripuberal period of transition. Four groups were formed according to a percentage of adult weight (AW), Group A with 2 ± 0.1 kg (40% of AW), Group B with 3 ± 0.1 kg (60% of AW), Group C with 3.5 ± 0.1 kg (70% of AW) and Group D with 4 ± 0.1 kg (80% of AW). The rabbits were euthanized according to the required weight for each group and ovaries, uterine horns, cervix and vagina were removed, in order to measure their length. Ovarian tissue fragments were obtained and processed by paraffin embedding for histological analysis using Masson´s trichrome stain. Primordial, primary, secundary and tertiary follicles were counted in the ovarian cortex and medulla. Regarding the length of the left and right ovary there were differences between Group A with respect to B, C and D (P ‹ 0.05). Regarding the length of the uterine horns there were differences between Group A with respect to groups B, C and D in both horns (P ‹ 0.05). Regarding the length of the cervix there was difference between Group B and the others (P ‹ 0.05) and the vagina showed difference between Group A and the other groups (P ‹ 0.05). With respect to the variable number of primordial follicles there was difference between Group A and the other groups (P ‹ 0.05). For primary and secondary follicles there were no differences between the four groups (P › 0.05). In tertiary follicles there were differences between Group A and the others (P ‹ 0.05). Based on the results of this study it is concluded that in New Zealand rabbits the histological and morphometric changes of the reproductive tract associated with body weight, are more dramatic in the transition range from 40% to 60% (groups A and B) of AW, hence, it is a critical period in the maturation of the female reproductive system.


REFERENCES

  1. MCNITT JI, PATTON NM, CHEEKE PR, LUKEFAHRSD. Rabbit Production. 8th ed. Danville USA: Interstate Publishers, 2000.

  2. TORRES LC, TORRES LR. Manejo en cunicultura. En: CARLOS BUXADÉ, coordinador. Zootecnia. Bases de Producción Animal. Madrid, España: Ed. Mundi-Prensa, 1996.

  3. ROMMERS JM, MEIJERHOF R, NOORDHUIZEN JP, KEMP B. Relationships between body weight at first mating and subsequent body development, feed intake, and reproductive performance of rabbit does. J Anim Sci 2002; 80:2036-2042.

  4. ALVARIÑO MR. Control de la Reproducción en el conejo. Madrid, España:Mundi Prensa, 1993.

  5. ALUJA AS. Animales de Laboratorio y la Norma Oficial Mexicana (NOM-062-ZOO-1999). Gac Med Méx 2002; 138:295-298.

  6. INTERNATIONAL COMMITTEE ON VETERINARY GROSS ANATOMICAL NOMENCLATURE. Nomina Anatomica Veterinaria. 5th ed. Knoxville, USA: World Association of Veterinary Anatomists, 2005.

  7. ESTRADA FE, PERALTA ZL, RIVAS MP. Manual de Técnicas Histológicas. México, DF: AGT Editor, 1982.

  8. HUTT KJ, MCLAUGHLIN EA, HOLLAND MK. Primordial follicle activation and follicular development in the juvenile rabbit ovary. Cell Tissue Res 2006; 326:809-822.

  9. MELVIN EJ, LINDSEY BR, QUINTAL-FRANCO J, ZANELLA E, FIKE KE, VAN TASSELL CP et al. Estradiol, luteinizing hormone, and follicle stimulating hormone during waves of ovarian follicular development in prepubertal cattle. Biol Reprod 1999; 60:405-412.

  10. BOBES RJ, PEREZ MM, GOMEZ Y, ROMANO MC. Metabolism of progesterone to estrogens and androgens by individual follicles of the goat ovary. Small Rum Res 2003; 47:233-242.

  11. ARAGONA P, PUZZOLO D, MICALI A, FERRERI G, BRITTI D. Morphological and morphometric analysis on the rabbit connective globet cells in different hormonal conditions. Exp Eye Res 1998; 66:81-88.

  12. BAI W, OLIVEROS-SAUNDERS B, WANG Q, ACEVEDODUNCAN M, NICOSIA S. Estrogen stimulation of ovarian surface epithelial cell proliferation. In Vitro Cell Dev Biol 2000; 36:657-666.

  13. KASUYA K. The process of apoptosis in folicular epithelial cells in the rabbit ovary, with special reference to involvement by macrophages. Arch Histol Cytol 1995; 58:257-264.

  14. MANDAL GE, ZUCKERMAN S. The number of normal and atretic ova in the Mature rat. J Endocrinol 1950; 6:426-435.

  15. FLAWS JA, HIRSHFIELD AN, HEWITT JA, BABUS JK, FURTH PA. Effect of Bcl-2 on the primordial follicle endowment in the mouse ovary. Biol Reprod 2001; 64:1153-1159.

  16. KAMWANJA LA, HAUSER ER.The influence of photoperiod on the onset of puberty in the female rabbit. J Anim Sci 1983; 56:1370-1375.




2020     |     www.medigraphic.com