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2010, Number 4

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Rev Mex Patol Clin Med Lab 2010; 57 (4)

Immature granulocytes: Reference values, using the sysmex XE-2100 blood counter

Sáenz FK, Narváez GL, Cruz M, Checa C

Language: Spanish
References: 13
Page: 163-169
PDF size: 180.91 Kb.


ABSTRACT

Introduction: Automated hemograms have undergone numerous technical innovations during the last years. The immature granulocytes count, based on differences in membrane composition and on their higher levels of RNA, is a new parameter in the differential blood cell count. Its advantages include a rapid processing time and low cost. For it to be used as a parameter for clinical analysis, it becomes essential to establish reference «healthy» values so potentially pathological levels can be identified. Material and methods: A descriptive epidemiological survey was performed on a convenience sample of 708 hemograms selected from adults of both sexes and ages ranging from 18 to 60 years submitted to Net-Lab S.A. (Quito-Ecuador), received as part of routine health checkups during January and February 2009. We used the blood counter Sysmex XE-2100®. Results: The average age of the subjects studied was 34.18 ± 11.7 years, and 64.4% of the subjects were males. Among this apparently healthy population, the reference absolute counts calculated for immature granulocytes ranged between zero and 0.03 x103/mm3, and the relative counts were between zero and 0.4%, without significant differences by gender. Conclusions: These results were similar to the values reported in a German study made with an «a priori» methodology in healthy blood donors in the University Hospital of Leipzig. Thus, the reference values we identified may be generalizable to other populations.


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